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目的：探讨细胞周期蛋白B2(Cyclin B2, CCNB2)在结直肠癌组织中的表达及其临床意义。方法：选择45 对结直肠癌组织及癌旁正常结直肠组织样本，分别采用实时定量PCR(qRT-PCR)方法和免疫组织化学技术检测CCNB2 的mRNA 和蛋白表达，并进一步分析CCNB2 的表达与结直肠癌临床病理特征之间的关系。结果：结直肠癌组织中CCNB2 mRNA 的表达显著高于癌旁正常结直肠组织，差异有统计学意义(P<0.001)，且CCNB2 的mRNA 表达与结直肠癌的肿瘤大小、浸润深度及TNM 分期显著相关 (P<0.05)，与年龄、性别、肿瘤位置、分化程度、脉管神经浸润、淋巴结转移和远处转移均无关(P>0.05)。45例结直肠癌标本中39 例表达(+ ~ +++)，6 例表达(-)。CCNB2 蛋白主要表达于结直肠癌细胞质中，少量见于细胞核。结直肠癌组织中CCNB2 蛋白的阳性表达率为86.7%，显著高于癌旁正常结直肠组织，并与患者的性别、年龄、分化程度和肿瘤转移均无显著相关性(P>0.05)，但与肿瘤分期、浸润程度均显著相关(P<0.05)。结论：CCNB2 在结直肠癌中呈异常高表达，且与结直肠癌的发生发展相关，有望作为结直肠癌的诊断和预后预测参考指标。

英文摘要:

Objective:To investigate the expression and clinical significance of Cyclin B2 (CCNB2) in colorectal cancer (CRC).Methods:The expression of CCNB2 in 45 pairs of CRC tissues and para-adjacent normal tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry. The relationship between the expression of CCNB2 and clinicopathologic characteristics was analyzed by SPSS 17.0.Results:The expression of CCNB2 mRNA in CRC tissue was significantly higher than that of the para-adjacent non-malignant tissues (P<0.001). It was significantly correlated with the tumor size, greater tumor depth, TNMstage and lymph node metastasis of CRC (P<0.05), but had no correlation with the age or gender of patients, tumor location, differentiation, venous and nervous invasion, lymph nodes metastasis and distant metastasis (P>0.05). The immuno-activity was detected mainly in the cytoplasm of colorectal cancer cells. The positive rate of CCNB2 protein expression in CRC tissues was 86.7%, markedly higher than that of the para-adjacent non-malignant tissues(P<0.001), and related to the tumor stage and invasion(P<0.05), but it had no correlation with the age or gender of patients, differentiation, metastasis (P>0.05).Conclusion:Overexpression of CCNB2 in colorectal cancer could be associated with the occurrence and progress of CRC and expected to be the diagnositic and prognostic indicator of CRC.

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