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目的：研究姜黄素的抗增殖作用是否依赖于其对Ets-1 表达的下调。方法：使用Ets-1 siRNA 对CFs 细胞Ets-1 基因进行沉默；Real-time PCR和western-blot 法测定各组细胞Ets-1 mRNA和蛋白的表达水平。结果：在mRNA和蛋白水平上，AngII明显增加CFs 细胞内Ets-1 的表达；使用siRNA 技术对Ets-1 进行沉默后，随着Ets-1 表达的降低及转录调节能力的下降，由AngII 诱导的CFs 细胞的增殖能力及增殖相关细胞因子分泌减少；姜黄素可以显著降低AngII 诱导的Ets-1 表达升高，并具有浓度依赖性（P<0.05）。说明姜黄素对AngII诱导的CFs 增殖的抑制作用可能依赖于其对Ets-1 表达的抑制作用。结论：Ets-1 siRNA 对Ets-1 进行基因沉默后，显示出抗增殖效用;姜黄素能够有效地抑制AngII诱导的CFs 细胞Ets-1 mRNA和蛋白的过表达；姜黄素的抗增殖作用可能依赖于其对Ets-1 基因的表达下调而得以实现的。

英文摘要:

Objective:To observe the inhibition effect of curcumin (Cur) on AngII-induced over-proliferation and overexpression of CTGF and PAI-1 in CFs and relation between its inhibitory effect and the suppressing expression of Ets-1.Methods:Ets-1 were measured by real-time PCR and western-blotting, respectively. After transfected with Ets-1 si-RNA, the cells were incubated with AngII (100 nmol/L) for indicated times. The cell activity was detected by MTT assay and the levels of mRNA and protein of CTGF, PAI-1, Ets-1 were measured by real-time PCR and western-blotting, respectively.Results:With the lower expression of Ets-1, the proliferation which were induced by AngII was decreased in CFs cells and the expression of CTGF and PAI-1 also declined. Curcumin can significantly reduced the expression of Ets-1 and the reduction was also concentration-related （P<0.05）Conclusion:Curcumin significantly inhibits AngII-induced overexpression of Ets-1 in the levels of mRNA and protein in CFs, and Ets-1 siRNA also has the same inhibitory effect as Cur. These results suggested that Cur's anti-fibrotic effect, in part, depends on its inhibitory effect on the overexpression of Ets-1 in AngII-stimulated CFs.

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