| 单文姣 郝一 廖鸿纯 张军 李亚林 杨姣 楚艳娥.LTF基因在鼻咽癌细胞系中生物学功能的初步研究[J].,2014,14(25):4843-4846 |
| LTF基因在鼻咽癌细胞系中生物学功能的初步研究 |
| The Preliminary Study of Biological Activity of LTF Gene in NPCDerived Cell Line |
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| DOI： |
| 中文关键词: LTF 鼻咽癌 真核表达 抑瘤基因 |
| 英文关键词: LTF NPC Eukaryotic expression Tumor inhibitory gene |
| 基金项目:湖南省教育厅资助科研项目(09C150) |
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| 中文摘要: |
| 目的：初步探讨野生型LTF基因在鼻咽癌细胞系中的生物学功能。方法：野生型LTF 导入鼻咽癌细胞系，G418 筛选，
RT-PCR和Western-blotting 分别在mRNA 和蛋白质水平进行验证，得到稳定表达LTF 基因的鼻咽癌细胞系。流式细胞术、平板
克隆形成实验和MTT 法分别检测细胞周期、细胞的克隆形成能力和细胞生长曲线。结果：成功导入LTF 并稳定表达的鼻咽癌
细胞系，G0-G1 期细胞百分比例明显增加(72.01 %vs 62.31 %)，G2-M 期细胞百分比例减少(6.26 %vs 10.81 %)；克隆形成能力降
低(39. 5%vs 59.7 %)，体外瘤细胞增殖能力降低(P < 0.05)。结论：LTF基因可阻滞细胞周期、抑制鼻咽癌细胞系的增殖能力和克隆
形成率，同时为进一步的体内试验研究奠定基础。 |
| 英文摘要: |
| Objective:To preliminarily study the biological characteristics of lactotransferrin (LTF) gene in nasopharyngeal cancinoma
(NPC) derived cell lines.Methods:Wild-typed LTF gene was transfected into NPC derived cell line. After G418 selection and confirmation
the expression at mRNA and protein level by RT-PCR and Western blotting analysis respectively, Cell cycle, cell proliferation
and colony formation ability were detected by flow cytometry(FCM) analysis, MTT analysis and colony formation assay respectively. Results:LTF expression in NPC cell line could block the cell cycle progression in G1 phase, leading to an increase in the number of cells in
G1 phase ( 72.01 %vs 62.31 %) and a decrease in the number of cells in G2/Mphase (6.26%vs 10.81%); And they had a much lower
colony efficiency ( 39.5%vs 59.7 %) and proliferated much more slowly(P < 0.05).Conclusion:Evidence showed that LTF blocked the
cell cycle progression in G1 phase and had an inhibitory effect on proliferation and colony formation efficiency of NPC cells. The obtainment
paved the way for further study on biological functions of LTF in vivo. |
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