| 任兆强 邢维聪 孙勇 刘菲 林存智.细胞因子IFN-γ , IL-2, TNF-α 和ADA对结核性和恶性胸腔积液鉴别诊断的价值（英文）[J].,2014,14(23):4471-4474 |
| 细胞因子IFN-γ , IL-2, TNF-α 和ADA对结核性和恶性胸腔积液鉴别诊断的价值（英文） |
| The Differential Diagnostic Value of Interferon-γ, Interleukin-2, Tumor NecrosisFactor αand Adenosine Deaminase in Tuberculous and Malignant Pleural Effusion |
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| DOI： |
| 中文关键词: 结核性胸腔积液 恶性胸腔积液 细胞因子 |
| 英文关键词: Tuberculous pleural effusion Malignant pleural effusion Cytokines |
| 基金项目:山东省医药科技发展计划资助项目（2009-194） |
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| 中文摘要: |
| 目的：探讨细胞因子γ- 干扰素（IFN -γ）、白介素-2（IL-2）、肿瘤坏死因子-α（TNF-α）和腺苷脱氨酶（ADA）对结核性和恶性
胸腔积液的鉴别诊断的价值。方法：以2012 年9 月至2013 年3 月期间在青岛大学医学院附属医院呼吸科及青岛胸科医院未经
治疗的胸腔积液患者为研究对象，其中恶性胸腔积液患者46 例，结核性胸腔积液患者42例。采用双抗体夹心酶联免疫吸附测定
法（ELISA）分别检测结核性和恶性胸腔积液患者中IFN -γ、IL-2、TNF-α及ADA的表达情况。并应用ROC曲线分析两组患者胸
腔积液中IFN -γ、IL-2、TNF-α及ADA的表达差异及意义。结果：结核性胸腔积液组IFN -γ、IL-2、TNF-α及ADA 的表达明显高于
恶性胸腔积液组，差异有统计学意义（t=8.118、8.126、8.066、7.221; P=0.000、0.000、0.000、0.000，P<0.001）；ROC曲线分析结果显示
胸腔积液中IFN -γ、IL-2、TNF-α及ADA的诊断临界值为201.45 pg/mL、41.91 pg/mL、21.55 pg/mL、33.78 U/L；诊断敏感度分别为
91.3 %、93.5 %、91.2 %、89.1 %；特异度分别为91.0 %、92.1 %、89.9 %、90.1 %。结论：胸腔积液中IFN -γ、IL-2、TNF-α及ADA 的表
达对结核性和恶性胸腔积液诊断与鉴别诊断具有重要参考价值。 |
| 英文摘要: |
| Objective:To explore the differential diagnostic value of interferon-γ(IFN-γ), interleukin-2 (IL-2), tumor necrosis
factor α(TNF-α), and adenosine deaminase (ADA) level in the tuberculous and malignant pleural effusions.Methods:A total of
eighty-eight specimens of pleural effusion were collected from 88 patients with pleural effusion in respiratorydepartment of affiliated
Hospital of Medical College of Qingdao University and Qingdao Thoracic Hospital from September 2012 to March 2013. Among them,
46 pleural specimens were diagnosed as malignant, and the others were diagnosed as tuberculous. Enzyme-linked immunosorbent assay
(ESISA) was used to detect the concentration of IFN-γ, IL-2, TNF-αand ADA in tuberculous and malignant pleural effusions. Receive
operating characteristic curve (ROC) analysis was used to analyze whether there are significant differences between the two groups of
patients with pleural effusion in concentration of IFN-γ, IL-2, TNF-αand ADA.Results:Tuberculous pleural effusion group of IFN-酌,
IL-2, TNF-αand ADA was significantly higher than that of malignant pleural effusion group, and the difference was statistically
significant ( t = 8.118, 8.126, 8.066, 7.221; P<0.05); ROC analysis showed that pleural effusion of IFN-γ, IL-2, TNF-αand ADA in the
diagnosis of the critical value of 201.45 pg/mL, 41.91 pg/mL, 21.55 pg/mL, 33.78 U/L, the sensitivity and specificity for IFN-γ, IL-2,
TNF-αand ADA in pleural fluid were 91.3 %, 93.5 %, 91.2 %, 89.1 % and 91.0 %, 92.1 %, 89.9 %, 90.1% , respectively.Conclusion:The IFN-γ, IL-2, TNF-αand ADA concentration in tuberculous and malignant pleural fluid are significant for the early diagnosis and
differential diagnosis. |
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