| 莫仁 彭景涛 马坚 张长存 阮渊 刘永超 周立斌 尹冰德 凡杰.MicroRNA-145 在肾癌中的临床意义及其分子机制探讨[J].,2014,14(21):4018-4021 |
| MicroRNA-145 在肾癌中的临床意义及其分子机制探讨 |
| Clinical Significance and Molecular Mechanismof MicroRNA-145in Renal Cell Carcinoma |
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| DOI： |
| 中文关键词: 肾癌 miRNA-145 癌基因 基因表达 |
| 英文关键词: Kidney cancer MiRNA-145 Oncogene Gene Expression |
| 基金项目:国家自然科学基金项目(81372753);上海市科学技术委员会医学引导项目(124119a2200) |
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| 中文摘要: |
| 目的：探讨miRNA-145 在肾癌中的临床意义及其分子机制。方法：通过实时定量PCR 检测和比较16例肾癌患者的肿瘤组
织及癌旁正常肾组织中miRNA-145 的表达，并分析miRNA-145 的表达水平与肾癌患者病理特征及临床分级的相关性。进一步采
用实时定量PCR 检测和比较肾癌细胞株786-O、769P 及正常肾细胞株HK2 中miRNA-145 表达量，通过转染miRNA-145 mimics
和阴性对照miRNA 至769P、786-O 肾癌细胞株后观察癌基因(bcl-2、e2f3、cdk6、ccnd1)mRNA的表达。结果：肾癌组织中miRNA-
145 的表达显著低于癌旁正常肾组织(P<0.05)，且与肾癌的直径、病理核分级及临床分级呈显著负相关(P<0.05)。肾癌细胞株
769P、786-O 中miRNA-145 的表达显著低于正常HK-2 肾细胞(P<0.05)，而转染miRNA-145 mimics 的769P、786-O 细胞中多个癌
基因(bcl-2、e2f3、cdk6、ccnd1)的mRNA表达均较阴性对照组显著降低(P<0.05)。结论：MiRNA-145 在肾癌中呈低表达，可能通过
调控多个癌基因的表达在肾癌的发生发展过程中发挥重要的作用。 |
| 英文摘要: |
| Objective:To investigate the clinical significance and molecular mechanism of miRNA-145 in renal cell carcinoma
(RCC).Methods:Quantitative real time PCR was used to examine and compare the miRNA-145 expression in 16 cases of RCC and adjacent
normal renal tissues, and further in RCC cell lines 786-O, 769P and normal HK2 cell lines. The correlation of miRNA-145 expression
with clinicopathological charateristics and clinical grading were analyzed. After being transfected with the miRNA-145 mimics, the
expression levels of multiple oncogenes (bcl-2, e2f3, cdk6, ccnd1) in RCC cell lines 786-O, 769P and normal HK2 cell line were examined
by QRT-PCR.Results:The miRNA-145 expression was downregulated in RCC tissue in comparison with that in the normal renal
tissue (P<0.05), which was negatively correlated with the diameter, pathological nuclear grading and clinical grading of RCC(P<0.05).
The miRNA-145 expression was significantly lower in RCC cell lines 786-O, 769P than that in the normal HK2 cell line (P<0.05). The
overexpression of miRNA-145 in 786-O, 769P cells significantly decreased the mRNA expression of multiple oncogenes (bcl-2, e2f3,
cdk6, ccnd1) (P<0.05).Conclusion:MiRNA-145 was downregulated in RCC, and may play an important role in the renal carcinogenesis
through regulating multiple oncogenes. |
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