| 李靖 谭晓华 何淼 刘如锦 王小波 杨磊.病毒巨噬细胞炎症蛋白-II对载脂蛋白B mRNA编辑酶催化样蛋白3G
和正常T 细胞表达分泌的调节活化蛋白表达的影响[J].,2014,14(21):4005-4008 |
| 病毒巨噬细胞炎症蛋白-II对载脂蛋白B mRNA编辑酶催化样蛋白3G
和正常T 细胞表达分泌的调节活化蛋白表达的影响 |
| vMIP-II Induce Apolipoprotein B mRNA-editing Catalytic Polypeptide-3Gand Regulated upon Activation Normal T Expressed and SecretedExpression |
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| DOI： |
| 中文关键词: 病毒巨噬细胞炎症蛋白-II HIV-1 抑制因子 载脂蛋白B mRNA编辑酶催化样蛋白3G 正常T 细胞表达分泌的调节活化
蛋白 |
| 英文关键词: Viral macrophage inflammatory protein-II HIV-1 restriction factor Apolipoprotein B mRNA-editing catalytic
polypeptide-3G Regulated upon activation Normal T expressed and secreted |
| 基金项目:国家自然科学基金项目(81071636)；浙江省科技厅国际合作重点项目(2009C14014)；
浙江省自然科学基金项目(Y12H160158)；浙江省重点科技创新团队计划资助（2011R50021） |
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| 中文摘要: |
| 目的：明确病毒巨噬细胞炎症蛋白-II (viral macrophage inflammatory protein-II，vMIP-II）对HIV-1 抑制因子：载脂蛋白
BmRNA 编辑酶催化蛋白3G(apolipoproteon B mRNA-editing catalytic polypeptide -3G，APOBEC 3G)和正常T 细胞表达分泌的调
节活化蛋白(regulated upon activation normal T expressed and secreted，RANTES) 表达的影响。方法：构建vMIP-II真核表达载
pEGFP-N3-vMIP-II，分别采用电穿孔和脂质体转染的方法将其转染至Jurkat 和293T细胞。荧光定量PCR检测vMIP-II 基因对
Jurkat细胞和293T 细胞内的抗艾滋病基因APOBEC3G和RANTES表达水平的影响。结果：测序结果显示成功构建了的vMIP-II
真核表达载体，荧光显微镜观察估计转染效率达到50%左右。与空载体组相比较，转染pEGFP-N3- vMIP-II组的Jurkat 细胞内的
APOBEC3G 和RANTES分别上调4.97 倍和7.31 倍，293T细胞内的APOBEC3G和RANTES 分别上调为5.73 倍和8.14 倍。结
论：vMIP-II基因不同程度的上调了Jurkat细胞和293T 细胞内的抗艾滋病基因APOBEC3G和RANTES的表达。 |
| 英文摘要: |
| Objective:To explore the impact of viral macrophage inflammatory protein-II (vMIP-II) on the expression levels of
HIV restriction factors apolipoproteinB mRNA-editing catalytic polypeptide-3G (APOBEC3G) and regulated upon activation, normal T
expressed and secreted (RANTES).Methods:The vMIP-II encoding gene was cloned into pEGFP-N3 vector to construct the recombinant
plasmids pEGFP-N3-vMIP-Ⅱ . The plasmids were transfected into Jurkat cells and 293T cells by electroporation and lipofectamine
2000TM reagent, respectively. The expression levels of APOBEC3G and RANTES were detected by quantitative real time polymerase
chain reaction (qRT-PCR).Results:The sequencing results verified the recombinant plasmids pEGFP-N3-vMIP-Ⅱ was successfully
constructed. The transfection efficiency reached about 50 % in both cell lines. Compared with the cells transfected pEGFP-N3 empty
vector, the cells transfected pEGFP-N3-vMIP-Ⅱ have increased APOBEC3G and RANTES expression levels in Jurkat cells (4.97 and
7.31 folds) and in 293T cells (5.73 and 8.14 folds).Conclusion:vMIP-II can induce the expression of host HIV -1 restriction factors of
APOBEC3G and RANTES in Jurkat cells and 293T cells. |
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