| 郝荣荣杨倩曹蔚肖会敏王四旺△.椒目及椒目仁油中α- 亚麻酸的含量测定研究*[J].,2014,14(20):3840-3843 |
| 椒目及椒目仁油中α- 亚麻酸的含量测定研究* |
| Study on the Determination of a-linolenic Acidin the ZanthoxylumBungeanumMaximand the SeedsOil fromZanthoxylumBungeanumMaxim* |
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| DOI： |
| 中文关键词: 椒目 椒目仁油 HPLC法 α-亚麻酸 |
| 英文关键词: ZanthoxylumbungeanumMaxim The seeds oil fromZanthoxylumbungeanumMaxim HPLC a-linolenic acid |
| 基金项目:陕西省"13115" 科技创新工程技术研究中心基金(S2010ZDGC105) |
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| 中文摘要: |
| 摘要目的：建立同时测定椒目及椒目仁油中α- 亚麻酸含量的HPLC 方法。方法：用固定相为Kromasil C18 柱(250 mm× 4.6 mm,
5滋m)，流动相为乙腈-1%醋酸溶液（90:10），检测波长为205nm，流速为1.0 mL·min-1，柱温：25℃，进样量：10 μL，测定椒目及椒目
仁油中α- 亚麻酸的含量；结果：α- 亚麻酸在（22～500）μg·mL-1浓度范围内线性关系良好,5 批椒目和椒目仁油中α- 亚麻酸的平
均含量分别为4.56%, 32.72%，平均回收率分别为99.87%,98.97%。结论：所建方法操作简便，准确可靠，重现性良好，可有效的控
制椒目及椒目仁油的质量。 |
| 英文摘要: |
| ABSTRACT Objective:To establish the HPLC method to determine the content of a-linolenic acid in the Zanthoxylum bungeanum
Maximand the seeds oil fromZanthoxylumbungeanumMaxim.Methods： With the stationary phase for Kromasil C18 column (250 mm
× 4.6 mm, 5 滋m), mobile phase of acetonitrile and 1%acetic acid solution (90:10), detection wavelength of 205 nm, flow rate of 1.0 mL
min -1, column temperature: 25 ℃ , sample quantity: 10 uL.to determine the content of a-linolenic acid in the Zanthoxylum bungeanum
Maximand the seeds oil from Zanthoxylum bungeanum Maxim. Results：Linear range of a-linolenic acid was (22～500）滋g·mL-1 , and
the average contents of a-linolenic acid were 4.56%and 32.72%, the average recoveries of a-linolenic acid were 99.87%and 98.97%for
Zanthoxylum bungeanum Maxim and the seeds oil from Zanthoxylum bungeanum Maxim respectively. Conclusion:The method is
simple, accurate, reliable and with good reproducibility, which can effectively control the quality of ZanthoxylumbungeanumMaximand
the seeds oil fromZanthoxylumbungeanumMaxim. |
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