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目的：季节气候的变化能够影响人体肠道的生理功能和病理变化，这在中医学的长期临床实践和现代流行病学调查中都有记载和证实，但是相关的机制并不清晰。因此本研究通过观察不同气候下大鼠空肠、回肠、结肠的IL-10 水平变化，从而从免疫功能的角度探讨季节气候变化对肠道功能的影响机制。方法：根据北京地区气象资料，大致可将一年之中气候划分为五种季节气候，分别由春、夏、长夏、秋和冬来代表；实验分为正常组和松果腺摘除模型组；分别把两组大鼠饲养五种正常自然季节气候下一个月，然后用ELISA 方法检测大鼠空肠、回肠和结肠的IL-10 水平。结果：①正常组空肠IL-10水平在长夏季节明显升高（P<0. 001）；② 回肠的IL-10 表达水平则是在长夏与冬季这两个季节含量最多（P<0.01, P<0.001）；③ 结肠IL-10 含量则在冬季呈现出高峰（P<0.01）。结论：季节气候变化对肠道功能的影响的机制与肠道免疫功能的季节性变化有关。具体表现在：① 肠道IL-10 水平是有季节气候变化规律的，而且空肠、回肠和结肠表现出来的季节气候规律是不相同的。②松果腺参与了肠道免疫功能气候调节过程。

英文摘要:

Objective:Epidemiological researches and traditional Chinese medicine studies showed that climate change had a great impact on guts' physiological and pathological functions. This study was designed to explore whether IL-10 levels in jejunum, ileum and colon follow climatic patterns.Methods:The climates of a year contain spring, summer, long summer, autumn and winter. The mice were divided into the control and pinealectomized groups, naturally fed for a month in each season. Finally, the levels of IL-10 in jejunum, ileumand colon were analyzed respectively by Elisa.Results:①Significantly, the level of IL-10 in jejunumwas highest in long summer (P<0.001). ② Compared with those of spring, summer and autumn, the level of IL-10 in ileum was obviously higher in long summer and winter （P<0.01, or P<0.001）respectively. ③ The level of IL-10 in colon increased much more in winter, compared with those of spring, summer, long summer and autumn（P<0.01）. The trend of IL-10 levels in intestinal tract of pinealectomized group was totally different from that of control group.Conclusion:① Strong climatic patterns of IL-10 level were observed in jejunum, ileum and colon. ② Pineal gland may play a role in the climatic patterns of IL-10 level in intestinal tract.

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