| 庞权 白素娟 闫佩毅 李文秀 金姝 冯会杰 杨杨.变应性鼻炎患者外周血中Tr1/Th3 细胞的检测和分析[J].,2014,14(18):3509-3512 |
| 变应性鼻炎患者外周血中Tr1/Th3 细胞的检测和分析 |
| Detection and Analysis of Tr1/Th3 cells in Peripheral Blood of Patientswith Allergic Rhinitis |
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| DOI： |
| 中文关键词: 鼻炎 变应性 外周血 Tr1 细胞 Th3 细胞 |
| 英文关键词: Rhinitis Allergic Peripheral blood Tr1 cells Th3 cells |
| 基金项目:上海市普陀区卫生系统自主创新资助项目(PTKW08-C01) |
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| 中文摘要: |
| 目的：检测变应性鼻炎(Allergic rhinitis，AR)患者和健康对照者外周血中IL-10+CD4+T 细胞、TGF-beta+CD4+T 细胞(分别代表
Tr1 细胞和Th3 细胞的特性)的比例，并探讨其在AR 发病中的意义，为AR的治疗提供临床参考。方法：分离19 例对粉尘螨过敏
的AR患者和19 例健康对照者外周血单个核细胞(PBMCs)，采用流式细胞术分别检测外周血中IL-10+CD4+T细胞、TGF-beta+CD4+T
细胞的比例。结果：同健康对照者相比，AR 患者外周血中IL-10+CD4+T 细胞的比例显著降低[(1.66± 0.48)%vs. (3.80.92)%，
t=-9.08，P＜0.01) ]，AR患者外周血中TGF-beta+CD4+T 细胞的比例降低[(1.92± 0.54)%vs.(4.76± 1.12)%，t=-9.94，P＜0.01) ]。结论：
外周血中IL-10+CD4+T(Tr1)细胞比例的降低可能是AR 发病的一个重要因素，提高AR 患者外周血中分泌IL-10 的Tr1 细胞的比
例可能在AR 的治疗中具有重要意义。外周血中TGF-beta1+CD4+T(Th3)细胞的比例显著降低，可能是AR发病的一个重要因素。但
TGF-beta1 与AR关系的研究较少，特别是外周血中TGF-beta1 水平与AR的关系研究较少，需进一步研究。 |
| 英文摘要: |
| Objective:To detect the proportion of IL-10+CD4+T cells and TGF-beta+CD4+T cells (which represent characteristics of
Tr1 cells and Th3 cells, respectively) in peripheral blood of allergic rhinitis (AR) patients and healthy controls, and to explore their
significance in the pathogenesis of AR in order to provide clinical references for the treatment of allergic rhinitis.Methods:Peripheral
blood mononuclear cells (PBMCs) of 19 AR patients who were sensed to Dermatophagoides farinae (Df) and 19 healthy controls were
isolated. The proportion of IL-10+CD4+T cells and TGF-beta+CD4+T cells of peripheral blood were detected by flow cytometry.Results:Comparing with healthy controls' the proportion of IL-10+CD4+ T cells in peripheral blood CD4+T cells significantly decreased and the
TGF-beta1+ CD4+T cells in peripheral blood significantly decreased (P<0.01).Conclusion:A lower proportion of IL-10+CD4+ (Tr1) cells in
peripheral blood may be an important factor in the pathogenesis of AR. Improve the proportion of IL-10 secreting Tr1 cells may be of
great significance in the treatment of AR. A lower proportion of TGF-beta1+CD4+T (Th3) cells in peripheral blood may be an important
factor in the pathogenesis of AR. However, few studies about the relationship between TGF-beta1 and AR, especially the relationship
between TGF-beta1 levels and AR in peripheral blood, further research is needed. |
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