| 续畅 刘泽洲 许可嘉 李健 杨美娟 牛建昭 唐炳华.高脂及MCD饮食诱导非酒精性脂肪性肝炎动物模型的比较[J].,2014,14(18):3451-3455 |
| 高脂及MCD饮食诱导非酒精性脂肪性肝炎动物模型的比较 |
| Comparison of High-fat Diet and Methionine-choline Deficient diet inducedby non-alcoholic Steatohepatitis Animal Model |
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| DOI： |
| 中文关键词: 非酒精性脂肪性肝炎 动物模型 比较 病理学 血清学 |
| 英文关键词: Non-alcoholic steatohepatitis Animal model Comparison Serology Pathology |
| 基金项目:国家自然科学基金面上项目（81273884）；高等学校学科创新引智计划资助项目（B07007） |
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| 中文摘要: |
| 目的：对高脂饮食诱发的大鼠NASH 模型与蛋氨酸胆碱缺乏饮食诱发的小鼠NASH 模型进行血清学及病理学比较，并初步
探讨两种模型的发病过程及机制。方法：高脂饮食喂养SD 大鼠8 周，蛋氨酸胆碱缺乏饮食喂养C57BL/6小鼠2 周，以制备NASH
模型。取材后，血清用比色法对TG、CHO、FPG 的含量进行检测，用放免法对FINS 的含量进行检测，并对HOMA-IR 指数进行计
算；肝组织制成石蜡切片及冰冻切片进行HE 及油红O染色，并根据“NAFLD 活动度积分”对各组肝组织进行NASH分级评估。
结果：高脂饮食大鼠血清中TG、CHO、FPG、FINS 的含量显著升高，经计算HOMA-IR 指数显著升高；MCD 小鼠血清中TG、CHO
的含量显著下降，FPG、FINS 的含量未发生显著性改变，经计算HOMA-IR 指数未发生显著性改变。HE 染色、油红O 染色及
NAFLD 活动度积分结果显示，高脂饮食大鼠及MCD小鼠的肝组织均已发展到NASH 阶段。结论：两种造模方法均可稳定的模
拟人类NASH疾病的血清学及病理学变化，其中高脂饮食诱发的大鼠NASH 模型可模拟人类的发病过程及机制，能够复制胰岛
素抵抗、氧化应激等人类全身代谢紊乱表现，在NASH 研究领域更占优势。 |
| 英文摘要: |
| Objective:Comparison of high-fat diet and methionine-choline deficient diet induced by non-alcoholic steatohepatitis
animal model in serology and pathology, and explore the pathogenesis and mechanism.Methods:SD rats feed high-fat diet and C57BL/6
mice feed methionine-choline deficient diet to become model of non-alcoholic steatohepatitis. Liver tissue and blood were harvested after
8 weeks (rats) and 2 weeks (mice), which were detected by TG, CHO, FPG, FINS, HE and oil-red O. At last, the rats were assessed by
NAFLD Activity Score (NAS) and HOMA-IR.Results:The levels of serum TG, CHO, FPG, FINS, HOMA-IR were significantly
increased in NASH rats. The levels of serumTG, CHOwere significantly decreased in NASH mice. The results of HE, oil-redO and NAS
show liver tissue of rats and mice have been developed to NASH.Conclusion:Two methods can be simulated human NASH disease in
serology and pathology, high-fat diet induced by NASH model can simulate the process and mechanism of human NASH disease, and
this model can copy insulin resistance and oxidative stress, so this method have more advantage in NASH research field. |
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