| 蒋平 孙秀佳 向正华 苏小平 李春波.丹参酮对于阿尔茨海默症大鼠颞叶iNOS，MMP-2 表达的
影响及机理研究[J].,2014,14(18):3401-3404 |
| 丹参酮对于阿尔茨海默症大鼠颞叶iNOS，MMP-2 表达的
影响及机理研究 |
| The Effect of Tanshinone on the Expression of iNOS, MMP-2 in Temporal ofAlzheimer's Disease Model Rats and Study on Mechanism |
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| DOI： |
| 中文关键词: 阿尔茨海默症 诱导型一氧化氮合成酶 基质金属蛋白酶 丹参酮II A |
| 英文关键词: Alzheimer's disease Nitric oxide synthase (iNOS) Matrix metalloproteinase II (MMP-2) Tanshinone II A |
| 基金项目:上海市卫生局科研项目(20114297)；上海交通大学医学院自然科学研究基金(11XJ21068) |
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| 中文摘要: |
| 目的：应用中药丹参酮(tanshinone II A，Tan II A)治疗AD大鼠，观察TanⅡ A 干预前后，AD大鼠学习记忆、颞叶中诱导型一氧
化氮合成酶(iNOS)、基质金属蛋白酶（MMP-2）表达的变化。方法：采用beta- 淀粉样蛋白（A beta）定向注射法建立AD大鼠模型，并使用
Tan II A 干预，通过避暗测试、real-time PCR和Western Blot 分别观察大鼠学习记忆能力、大鼠颞叶MMP-2、iNOS 两者的mRNA
及蛋白表达的变化。应用SPSS13.0 进行统计学分析。结果：与假手术组相比，AD 组的平均潜伏期缩短（P＜0.01），平均错误次数
增加（P＜0.01），差异均有统计学意义。颞叶内iNOS、MMP-2 mRNA 表达均显著增高（P＜ 0.01, P＜0.01）；两蛋白的表达均显著增
高（P＜0.01, P＜0.01）。与AD组相比，Tan IIA 组的平均潜伏期延长（P＜0.01），平均错误次数减少（P＜0.01），差异均有统计学意
义。颞叶内iNOS、MMP-2 mRNA表达均显著下降（P＜0.05, P＜0.05），两蛋白的表达均显著下降（P＜0.01, P＜0.01）。结论：Tan II
A 干预可显著降低AD 大鼠颞叶中iNOS、MMP-2 mRNA及蛋白的表达，显著改善AD 大鼠的学习记忆能力。其作用机制可能是
通过降低A-beta诱导的iNOS及MMP-2 的表达，抑制氧化应激损伤来完成。 |
| 英文摘要: |
| Objective:To investigate the influence of tanshinone IIA (Tan II A) on the learning and memory ability, the expression
of the inducible nitric oxide synthase (iNOS), matrix metalloproteinase II (MMP-2) in temporal of Alzheimer's disease (AD) model rats.Methods:AD model rats were established by direct beta-amyloid protein (A beta) injection method. For Tan IIA intervention test, the learning
and memory ability of AD rats were observed by passive avoidance task test, the expression of iNOS, MMP-2 at mRNA and protein
levels in temporal region were compared by real-time PCR and Western Blot respectively. Using SPSS 13.0 to statistic analyse the data.Results:Compared with sham-operation group, the average latency time of passive avoidance task test in AD group was decreased (P<
0.01), and the average wrong times of passive avoidance task test in AD group was significantly increased (P< 0.01). Both mRNA and
protein expressions of iNOS, MMP-2 in temporal were significantly increased (mRNA:P< 0.01, P< 0.01; protein: P<0.01, P<0.01). Compared
with AD group, the average latency time in Tan II A group was significantly increased (P<0.01), and the average wrong times in
Tan IIA group was significantly decreased(P<0.01). Both mRNA and protein expressions of iNOS, MMP-2 of temporal in Tan IIA group
were significantly decreased respectively (mRNA:P<0.05, P<0.05; protein: P< 0.01, P< 0.01).Conclusion:Tan II A can effectively enhance
the ability of learning and memory of AD rats, significantly decrease mRNA and protein expressions of iNOS, MMP-2 in temporal
of AD rats. This mechanism is probably inhibiting iNOS and MMP-2 expressions induced by A-beta in AD rats through suppressing
oxidative injury. |
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