文章摘要
韩蕃颉1 张红军1△ 刘希光1 宋浩1 冯龄鑫1 王相2.凋亡素2 配体对放射线诱导95-D细胞凋亡的影响的研究*[J].,2014,14(17):3244-3247
凋亡素2 配体对放射线诱导95-D细胞凋亡的影响的研究*
Study on the Effects of Opoptosis-2 Ligand on Apoptosis of 95-D LungCancer Cells that Radiation Induced*
  
DOI:
中文关键词: 凋亡素2 配体  细胞凋亡  细胞增殖  放射线疗法
英文关键词: Apoptosis-2 ligand  Cell apoptosis  Cell proliferation  Radiation
基金项目:卫生部科研课题(W2012FZ078)
作者单位
韩蕃颉1 张红军1△ 刘希光1 宋浩1 冯龄鑫1 王相2 1 青岛大学附属医院肿瘤科山东青岛2660032 青岛大学附属医院放疗中心山东青岛266003 
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中文摘要:
      摘要目的:探讨凋亡素2 配体(Apo-2L)对放射线诱导肺癌95-D细胞凋亡的影响。方法:MTT 法检测不同浓度凋亡素2 配体在 体外对肺癌95-D细胞的抑制率,将细胞分为4组,对照组、凋亡素2 配体组、单纯照射组、凋亡素2 配体+放射照射组,流式细胞 仪检测各组凋亡率及细胞周期。结果:凋亡素2 配体对95-D 细胞的体外抑制作用明显,随着药物浓度的增大及时间的延长,抑制 率明显增高(P<0.05)。流式细胞术显示凋亡素2 配体与放射线联用能够使95-D 细胞的凋亡率提高,与单用凋亡素2配体组及单 纯放疗组相比,凋亡率差异显著(P< 0.05)。结论:凋亡素2 配体在体外具有抑制95-D 细胞增殖的作用并能够促进细胞的凋亡,同 时凋亡素2 配体联合放射线可以明显提高肺癌95-D细胞的凋亡率。
英文摘要:
      ABSTRACT Objective:To investigate the effect of opoptosis-2 ligand on apoptosis of 95-D cells that radiation induced. Methods:The effect of the different concentration of the apoptosis-2 ligand on cellular proliferation inhibition rate of 95-D cells was detected by MTT method; The effect of combined apoptosis-2 ligand with radiation on the cell apoptosis and the cell cycle was detected by flow cytometry. Results:The cellular proliferation inhibition rate of apoptosis-2 ligand on 95-D cells was related with the drug concentration and action time (P<0.05). The combined application of apoptosis-2 ligand and radiation could raise the cellular proliferation inhibition rate of 95-D cells, compared with that in the control group and apoptosis-2 ligand or radiation alone(P<0.05). Conclusion:Apoptosis-2 ligand can inhibit the proliferation of 95-D cells culture and combined with apoptosis-2 ligand and radiation can raise the cellular proliferation inhibition rate of 95-D cells.
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