| Objective: To investigate the anti-tumor effects and the effect condition of the chimera level and GVHD of
transplanting MHC half-matched bone marrow cells combined with spleen cells in a murine model bearing colorectal cancer.Methods:Female (BALB/exC57BI/6)FI mice (CB6FI, H-2Kb/d) were subcutaneously inoculated with CT26 cells to develop a solid tumor model.
The syngenic, half-matched, and mismached donor bone marrow cells were from healthy female FI, maleC57BL/6 (H-2Kb), and male
C3H (H-2K)mice, respectively. The tumor weights were measured after transplantation. Meanwhile, the testing-group which received
chemotherapy, transplanting MHC half-matched bone marrow cells combined with spleen cells was used to analyze the chimera level.
And GVHD were observed after the transplantation.Results:The tumor weights of mice undergone chemotherapy pretreatment, bone
marrow cells combined with spleen cells were significantly decreased compared with those of the mice receiving chemotherapy alone
(P＜0.05). The mice which received chemotherapy, transplanting MHC half-matched bone marrow cells combined with spleen cells
attained donor implanted completely in 3 weeks after transplantation. Then began to fall ill at 10 days post-transplantation, GVHD
symptoms including decreased appetite, tired,instability of gait, unhairing, lose weight, diarrhea.Conclusion:The tumor weights of mice undergone chemotherapy pretreatment, bone
marrow cells combined with spleen cells were significantly decreased compared with those of the mice receiving chemotherapy alone
(P＜0.05). The mice which received chemotherapy, transplanting MHC half-matched bone marrow cells combined with spleen cells
attained donor implanted completely in 3 weeks after transplantation. Then began to fall ill at 10 days post-transplantation, GVHD
symptoms including decreased appetite, tired,instability of gait, unhairing, lose weight, diarrhea. |
