| 单冬凯 邹路路 宋晓伟 赵仙先 荆清.小鼠不同组织及缺氧损伤后的大鼠心肌细胞中RIP3的表达[J].,2014,14(16):3001-3004 |
| 小鼠不同组织及缺氧损伤后的大鼠心肌细胞中RIP3的表达 |
| Expression Profile of Receptor Interacting Protein 3 in Different MouseTissues and Rat's Myocardial Cells with Hypoxia Injury |
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| DOI： |
| 中文关键词: 丝氨酸/ 苏氨酸蛋白激酶家族 心肌细胞 缺氧 坏死 |
| 英文关键词: Receptor interacting proteins(RIPs) Myocardial cells Hypoxia Necrosis |
| 基金项目:国家自然科学基金项目(81130005，31100830) |
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| 中文摘要: |
| 目的：检测小鼠组织中受体相互作用丝氨酸/ 苏氨酸蛋白激酶家族(RIPs)表达谱，并检测RIP3 在大鼠心肌细胞缺氧损伤后
的表达。方法：① 采用荧光实时定量PCR 分别检测RIPs家族基因在小鼠组织(心、肝、肺、肾、脑、小肠、骨骼肌、脾和主动脉)中的
mRNA 表达谱，并采用Western blot进一步检测RIP3 在小鼠组织的蛋白表达谱。② 将培养的大鼠心肌细胞分为缺氧组和对照
组，缺氧组置于缺氧环境中培养48 h，采用western blot 检测其中RIP3 的表达变化。结果：① mRNA水平：RIP1 mRNA在脑组织
中表达最高，心脏、肺、肾、骨骼肌较低；RIP2在心脏和肺表达量较其他组织高；RIP3在肠中表达较其他组织高出4 倍以上，脑组
织中未检测到RIP3 表达；RIP4 的表达以肺最高，而骨骼肌、脑和血管中表达量低。② 蛋白水平：在小鼠组织中，RIP3 表达以脑、骨
骼肌中最高，心脏、肝、肺中表达较低。③ 培养的大鼠心肌细胞中，缺氧组心肌细胞的RIP3 表达量显著高于对照组(P<0.05)。结论：
RIPs在小鼠组织中呈现差异表达，而在培养的大鼠心肌细胞缺氧损伤后RIP3表达升高。 |
| 英文摘要: |
| Objective: To detect the expression profile of receptor interacting proteins (RIPs) in mouse tissues, and to detect the
expression of RIP3 in rat's myocardial cells with hypoxia injury.Methods:① We detected the expression profile of RIPs family in mouse
tissues (heart, liver, lung, kidney, brain, intestine, skeletal muscles, spleen and aorta) by real-time fluorescent quantitative PCR, then
detected the expression profile of RIP3 protein. ② The myocardial cells of rat were divided into hypoxia group and control. We treated
the first group for 48 h on hypoxia to obtain hypoxia injured models, then quantitated the expression of RIP3 by western blot.Results:①
On mRNA level, the expression of RIP1 was highest in brain, but lower in heart, lung, kidney and skeletal muscle; the expression of RIP2
was higher in heart and lung than other tissues; RIP3 in the intestine was 4 times higher than other, but it was very low expression in
brain; the expression of RIP4 was highest in lung, and was lowest in skeletal muscle, brain, spleen, and vessel. ② Western blot results
showed the expression of RIP3 at protein level. It was higher expressed in brain, skeletal muscle, however, lower in heart, liver and lung.
③ After treated on hypoxia, RIP3 of hypoxia group was significantly higher than control in the cultured rat myocardial cell (P<0.05).Conclusion:The expression of RIPs represented various level in mouse tissues. RIP3 increased in rat myocardial cells under hypoxia
injured condition. |
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