| 马靖华 王茂 刘晓莺 赵景杰 屈玲.ICU耐甲氧西林金黄色葡萄球菌的基因检测与感染控制[J].,2014,14(12):2316-2320 |
| ICU耐甲氧西林金黄色葡萄球菌的基因检测与感染控制 |
| Detection of Genes for Methicillin-Resistant Staphylococcus Aureus andMonitoring of Infection on the ICU |
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| DOI： |
| 中文关键词: 耐甲氧西林金黄色 葡萄球菌葡萄球菌染色体基因盒 多重聚合酶链反应 耐药性 |
| 英文关键词: Methicillin-resistant Staphylococcus Staphylococcus aureus chromosomal gene cassette Multiplex PCR Drug resistance |
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| 中文摘要: |
| 目的：研究耐甲氧西林金黄色葡萄球菌（MRSA）SCCmec 基因分型情况并对其耐药谱进行分析。方法：收集临床标本522
例，应用多重PCR法对MRSA 进行SCCmec 基因分型，采用全自动微生物鉴定药敏分析仪进行细菌的鉴定及药敏试验，部分药
敏试验采用K-B法。结果：522 例中分离出146 例MRSA，其中10 株为SCCmec I型(6.84％)，29 株为SCCmec II 型(19.86％)，103
株为SCCmecⅢ型(70.55％)，未分型4株(2.74％)。MRSA 分离株对奎奴普汀/达福普汀、替考拉宁、复方磺胺甲恶唑、万古霉素和
利奈唑胺敏感，SCC mecII 型与SCC mecIII型对氯霉素的耐药率分别为27.59%和11.65%，对利福平的耐药率分别为13.79%和
2.91%，存在明显的差异性（P＜0.05），其余均呈高水平耐药。146 例MRSA 患者中治愈52 例，占35.62 %；感染相关死亡者12例，
占8.22%。结论：ICU 耐甲氧西林金黄色葡萄球菌的基因检测以SCC mecIII型为主，且对抗菌药物呈多药耐药。 |
| 英文摘要: |
| Objective:To study the genotypes of staphylococcal cassette chromosome mec (SCCmec) and analyze the antimicrobial
resistance spectrum of meticillin-resistant Staphylococcus aureus (MRSA).Methods:522 cases with the clinical specimens were collected
and the grnotypes of SCCmec were determined by the multiplex PCR. Bacterial identification and antimicrobial susceptibility testing
were performed by full automatic microorganism identification and antimicrobial susceptibility testing system. Part of drug susceptibility
tests were performed by Kirby-Bauer method.Results:146 strains of MRSA were collected in the 522 samples, 10 (6.84%) strains
belonged to SCCmec I, 29 (19.86%) strains belonged to SCCmec II, 103 (70.55%) trains belonged to SCCmec Ⅲ , 4 (2.74 %)
strains belonged to unclassified. The resistance rates of MRSA to Kuinuputing /dalfopristin, teicoplanin, sulfamethoxazole, vancomycin
and linezolid were 0. The resistance rates of SCC mecII and SCC mecIII genotypes of MRSA to chloramphenicol were 27.59% and
11.65%, and the rifampicin were 13.79% and 2.91% with statistically significant differences (P<0.05). 52 (35.62%) got cured and 12
(8.22%)died of the infection.Conclusion:SCC mecIII is the major genotype of MRSA which was collected from ICU and presented as
the multidrug-resistance to antibiotics. |
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