| 李健楠 吴畏 冉明梓 杨岑 欧阳鹏荣 董海龙.蓝斑区去甲肾上腺素能神经元在Orexin 促麻醉觉醒中的作用研究[J].,2014,14(12):2239-2241 |
| 蓝斑区去甲肾上腺素能神经元在Orexin 促麻醉觉醒中的作用研究 |
| Activation of Norepinephrine Neurons in Locus Coeruleus Mediates thePromotion Effect of Orexin on Anesthesia Emergence |
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| DOI： |
| 中文关键词: Orexin 蓝斑 去甲肾上腺素 微注射 觉醒 |
| 英文关键词: Orexin LC Norepinephrine Microinjection Emergence |
| 基金项目:国家自然科学基金项目(30972853；81128005；81371510) |
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| 中文摘要: |
| 目的：探讨蓝斑区(LC)去甲肾上腺素能神经元在orexin 促麻醉觉醒中作用。方法：应用异氟烷对成年SD 大鼠进行麻醉，15
分钟后，将SD 大鼠随机分为6 组，分别注射orexin-A/B (100pmol/0.3 滋L) 及其溶剂saline (0.3 滋L)；orexin I 型受体拮抗剂
SB334867/ II型受体拮抗剂TCS-OX2-29(20 滋g/0.3 滋L及其溶剂DMSO(0.3 滋L)，通过观察大鼠翻正反射的消失和恢复时间，研究
蓝斑区微注射orexin 及其拮抗剂对异氟烷麻醉的诱导和觉醒的影响。结果：蓝斑区(LC)微注射四种试剂或其溶剂均对SD 大鼠异
氟烷麻醉的诱导时间无明显影响；蓝斑区(LC)微注射orexin-A 能缩短SD 大鼠异氟烷麻醉觉醒时间(P<0.001)，而微注射orexinI
型拮抗剂SB334867 能延长觉醒时间(P<0.001)；orexin-B、orexin II型受体拮抗剂TCS-OX2-29 对大鼠异氟烷麻醉的觉醒无明显影
响。结论：蓝斑区(LC)的去甲肾上腺素能神经元介导了orexin 的促麻醉觉醒作用。 |
| 英文摘要: |
| Objective:To investigate the role of norepinephrine neurons of the locus coeruleusin in the promotion effect of orexin
in emergence of isoflurane anesthesia.Methods:Isoflurane (ISO) was used to anesthetize adult male SD rats, 15 minutes later, the rats
were assigned into six groups, Orexin-A, orexin-B (100pmol/0.3 滋L) and their solvent saline (0.3 滋L), orexin receptor I antagonist
SB334867, orexin receptor II antagonist TCS-OX2-29 (20 滋g/0.3 滋L for both) and their solvent DMSO (0.3 滋L) were bilaterally microinjected
into LC, respectively. The time of the loss of righting reflex (LORR) following anesthesia was recorded as induction time and
the recovery of righting reflex (RORR) from cease of anesthesia were recorded as emergence time.Results:There was no different effect
on the induction time of ISO by any of above six microinjections(P>0.05). Microinject orexin-A into LC significantly shortened the emergence
time and orexin-A receptor I antagonis SB334867 prolonged the emergence time of ISO anesthesia (P<0.001).Conclusion:The
norepinephrine neurons of LC at least partly mediated the promotion effect of orexin in anesthesia emergence. |
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