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目的：研究正常雌性Sprague-Dawle（SD）大鼠不同性发育阶段及雌激素诱导性早熟后下丘脑Lin28a 和Lin28b 的表达及意义。方法：1）于雌性SD大鼠出生后15 日（postnatal day 15，PND15）、PND23、PND35 荧光实时定量PCR分析下丘脑Lin28a 和 Lin28b mRNA 的表达，同时以ELISA 法检测血清黄体生成素（LH）和雌二醇（E2）水平变化；2）苯甲酸雌二醇（estradiol benzoate， EB）诱导的性早熟大鼠随机分为EB-1 组和EB-2 组，分别于阴道开口（vaginal opening，VO）时及PND56 两个时间点处死，相应的发育阶段的大鼠用无菌芝麻油（sesame oil，OIL）作为对照分为OIL-1 和OIL-2 组；荧光实时定量PCR 分析下丘脑Lin28a 和 Lin28b mRNA 的表达，ELISA 法检测LH 和E2水平变化，并观察性早熟对大鼠阴道开口、体重、顶臀径、胫骨长等生长发育指标的影响。结果：1）PND15、PND23 和PND35组下丘脑Lin28a 和Lin28b mRNA 表达、血清LH 和E2水平无统计学差异（P＞0.05）；2） EB-1 组下丘脑Lin28a 和Lin28b mRNA表达高于OIL-1 组（P＜0.05），血清LH 和E2水平与OIL-1 组相比无统计学差异（P＞ 0.05）；EB-2 组下丘脑Lin28a 和Lin28b mRNA 表达高于OIL-2 组（P＜ 0.05），血清LH 和E2水平低于OIL-2 组（P＜ 0.05）；3）与 OIL-2 组比较，EB-2 组VO时间明显提前（P＜0.01），体重、顶臀长、胫骨长差异无统计学差异（P＞ 0.05）。结论：外源性雌激素引起的性早熟可能导致下丘脑Lin28a 和Lin28b 表达异常。

英文摘要:

Objective:To investigate expression and significance of Lin28a and Lin28b mRNA in hypothalamus of female SD rats at various stages of development and precocious puberty induced by estrogen.Methode:1）Analyze expression of Lin28a and Lin28b mRNA in hypothalamus of SD rats with Real-time PCR on postnatal day 15（PND15）, PND23 and PND35, meanwhile examine level of serumLH and E2 with ELISA；2）EB（estradiol benzoate）-induced precocious puberty rats are divided into EB-1 and EB-2 groups and are respectively sacrificed at the time of vaginal opening（VO）and PND56，at the same time respectively controlled with OIL-1 and OIL-2 groups which are induced by sesame oil（OIL）. Analyze expression of Lin28a and Lin28b mRNA in hypothalamus with Real-time PCR， examine level of serumLH and E2 with ELISA and observe influence of precious puberty on VO, weight, crown-rump length，tibia length at the same time.Results:1）Expression of Lin28a and Lin28b mRNA in hypothalamus and level of serum LH and E2 among PND15, PND23 and PND35 groups are not statistically significant（P＞0.05）; 2）Expression of Lin28a and Lin28b mRNA in EB-1 is higher than OIL-1（P＜0.05）, while level of serumLH and E2 between two groups is not statistically significant（P＞0.05）; Expression of Lin28a and Lin28b mRNA in EB-2 is higher than OIL-2 （P＜0.05）, meanwhile level of serum LH and E2 between two groups is statistically significant（P＜0.05）; 3）Time of VO in EB-2 group is earlier than OIL-2 group（P＜0.01）, while weight, crown-rump length, tibia length between two groups is not statistically significant（P＞0.05）.Conclusion:Precious puberty induced by Exogenous Estrogen could result in abnormal expression of Lin28a and Lin28b mRNA in hypothalamus.

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