| 袁媛 张利军 王超 郑鹏飞 张秀敏 叶菁 冯旭阳.脂多糖通过自噬作用促进氧化低密度脂蛋白诱导的泡沫细胞的形成[J].,2014,14(12):2205-2209 |
| 脂多糖通过自噬作用促进氧化低密度脂蛋白诱导的泡沫细胞的形成 |
| Lipopolysaccharide Enhances ox-LDL-induced Foam Cell Formationthrough Autophagy |
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| DOI： |
| 中文关键词: 脂多糖 自噬 泡沫细胞 ox-LDL |
| 英文关键词: Lipopolysaccharide Autophagy Foamcell Ox-LDL |
| 基金项目:国家自然科学基金项目（81070248，81070249）；陕西省自然科学基础研究计划项目（2013JQ4020） |
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| 中文摘要: |
| 目的：研究脂多糖（LPS）促进氧化低密度脂蛋白（ox-LDL）诱导的泡沫细胞形成的机制。方法：人源性THP-1 细胞的培养，经
ox-LDL 诱导形成泡沫细胞。采用油红O 染色鉴定泡沫细胞的形成，免疫荧光和Western blot 方法检测自噬活性，观察自噬作用对
泡沫细胞脂质沉积的影响。结果：①经形态学观察，脂多糖可以促进ox-LDL 诱导的泡沫细胞的形成。②脂多糖可以激活自噬作
用，并且自噬活性在16h 达到最强。③脂多糖可以增强自噬激活剂雷帕霉素（Rap）的促自噬作用（P＜0.05），并且削弱自噬抑制剂
3- 甲基腺嘌呤（3-MA）的作用。④Rap 单独作用不能影响泡沫细胞中脂质的累积，然而脂多糖能够增强Rap 的作用，显著促进脂滴
在泡沫细胞中的累积（P＜0.05）；3-MA 可以抑制基础水平和脂多糖诱导后泡沫细胞中脂滴的积累。结论：脂多糖通过增强自噬作
用促进泡沫细胞的形成。 |
| 英文摘要: |
| Objective:To investigate the mechanism of LPS in the ox-LDL-induced foam cell formation.Methods:Human THP-1
cells were cultured and differentiated into foam cells by the addition of ox-LDL. The foam cell formation was evaluated by Oil red O
staining. The autophagic activity was determined by immunofluorescence and Western blot analysis.Results:① By observing the
morphology of the foam cells, it was found LPS enhanced ox-LDL induced lipid accumulation during the foam cell formation. ② LPS
induced autophagy in human macrophages-derived foam cell formation. Moreover, the time-course (0h, 8h, 16h, or 24h) experiments
revealed that the percentage of cells expressing autophagosomes was maximal at 16hr after LPS stimulation. ③ Autophagic activity in
human THP-1 macrophages was either stimulated with Rap or inhibited by 3-MA. LPS could augment the activity of autophagy after Rap
and prevent the role of 3-MA. ④ Lipid accumulation increased by the exposure to LPS, and the increase was enhanced by Rap. In
contrast, inhibition of autophagy with 3-MA prevented both the basal and the LPS-induced lipid accumulation. Rapalone did not alter the
level of total cholesterol or triglyceride, while inhibition of autophagy with 3-MA decreased triglyceride in macrophages either treated
with LPS or not (P<0.05).Conclusion:LPS augments the foamcell formation through autophagy. |
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