| 邵宏超1 葛嫣然1 马建英2 刘志英1 邢达勇1 曹凤英1 田华1.caspase-2 与p53 在兔视网膜缺血再灌注损伤中的表达
及rh-bFGF对其表达的影响*[J].,2014,14(10):1844-1847 |
| caspase-2 与p53 在兔视网膜缺血再灌注损伤中的表达
及rh-bFGF对其表达的影响* |
| The Expression of Caspase-2 and p53 in Rabbit Retina inIschemia-Reperfusion Injury and after Rh-bFGF Treatment* |
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| DOI： |
| 中文关键词: 重组人碱性成纤维细胞生长因子 视网膜 缺血再灌注 caspase-2 P53 |
| 英文关键词: Recombinant human basic fibroblast growth factor Retina Ischemia-reperfusion Caspase-2 P53 |
| 基金项目:唐山市科技计划资助项目（12140209A-45） |
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| 中文摘要: |
| 摘要目的：探讨兔视网膜缺血再灌注损伤（RIRI）中caspase-2、P53 蛋白表达及重组人碱性成纤维细胞生长因子（Recombinant human
basic fibroblast growth factor，rh-bFGF）对其影响。方法：将104 只健康纯种大耳白兔随机分为正常组、缺血再灌注模型组、
rh-bFGF治疗组，各组均将左眼作为实验眼。缺血再灌注模型组和rh-bFGF治疗组按照不同再灌注时间各分为1h、6h、12h、24h、
48h、72h 6个时间段。后两组兔双眼做缺血再灌注损伤模型后，缺血再灌注模型组给予平衡盐溶液，rh-bFGF 治疗组给予rh-bFGF
药物治疗。免疫组织化学法检测视网膜组织中caspase-2、P53 蛋白的表达变化。结果：caspase-2、P53在正常视网膜组织中几乎不
表达，在缺血再灌注1h 开始表达，24h 达到高峰，48h 开始减弱，后逐渐下降，rh-bFGF治疗组各时间点观察指标变化趋势与缺血
再灌注模型组基本相似。rh-bFGF 治疗组与模型组比较，两种蛋白表达均明显减弱，再灌注6-72h 各时段差异有显著统计学意义
（P<0.05）。结论：rh-bFGF通过抑制视网膜缺血再灌注时caspase-2、P53 基因的表达减少视网膜细胞的凋亡，保护视网膜组织。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate The expression of caspase-2 and P53 in rabbit retina in ischemia-reperfusion injury and after
rh- bFGF treatment. Methods:One hundred and four healthy adult rabbits without eye diseases were randomly divided into normal
contral group,ischemia-reperfusion model group and rh-bFGF treatment group. Ischemia-reperfusion model group and rh-bFGF treatment
group were subdivided into 1hours, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours after reperfusion groups.In ischemia-reperfusion
model group and rh-bFGF treatment group, the dual eyes estabilished the ischemia-reperfusion injury models, saline was given to rabbit
in ischemia-reperfusion model group and rh-bFGF drug therapy performed in rh-bFGF treatment group.Immunohistochemical method
was used to measure changes of caspase-2, P53 protein levels in retinal tissues. Results:No expression of caspase-2, P53 positive cells
were found in normal contral group. At the ischemia-reperfusion model group, the expression of caspase-2, P53 began to increase at 1
hours after reperfusion. At 24 hours after reperfusion the expression reached the peak, kept expressing strongly at the 48th hour , and
decreased at the 72nd hour. The treatment groups of rh-bFGF had the same trend with the ischemia-reperfusion model group in each
index.rh-bFGF treatment group being compared with ischemia-reperfusion model group, caspase-2,P53 expression was significantly
reduced.There was statistical significance of the expression of caspase-2, P53 between the ischemia-reperfusion model group and
rh-bFGF treatment group 6-72 hours after reperfusion (P<0.05). Conclusion:Caspase-2,P53 protein had taken part in the RIRI.rh-bFGF
could cut the expression of caspase-2, P53 protein,and protect the retina tissue. |
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