| 李建平 王洪玲 高玉丽 丁弦 夏晨蕾 贾萍萍 张强 王爽玉
阎潇 刘文 张月 姚如永 袁晓.周期性张应力刺激下成肌细胞钙网蛋白的表达及变化[J].,2014,14(9):1657-1661 |
| 周期性张应力刺激下成肌细胞钙网蛋白的表达及变化 |
| Expression and Change of CRT in Stretch -inducedApoptosis of Myoblast |
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| DOI： |
| 中文关键词: 张应力 钙网蛋白 细胞凋亡 功能矫形 成肌细胞 |
| 英文关键词: Tensile stress Calprotectin Cell apoptosis Functional orthopedics Myoblast |
| 基金项目:国家自然科学基金项目（31170891） |
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| 中文摘要: |
| 目的：检测成肌细胞钙网蛋白(CRT)在循环拉伸应力刺激下的表达变化。方法：体外构建面颌部成肌细胞力学刺激模型。加
力组以0.5 赫兹的加载频率和10%细胞拉伸变形幅度对细胞进行加力培养1 h, 6 h, 12 h, 24 h，运用实时荧光定量PCR 技术跟
Western Blot技术分别检测在周期性张应力作用下成肌细胞CRT 在基因水平及蛋白水平的表达变化。结果：当对细胞加力6 h
后，CRT mRNA及蛋白表达量开始增多，加力12 h后CRT mRNA及蛋白表达量到达最多（P＜0.01），加力0h 组与加力12 h组之
间差异有显著的统计学意义（P＜0.01）。结论：持续的周期性张应力刺激下CRT mRNA 及蛋白表达增加。 |
| 英文摘要: |
| Objective:To investigate the changes of Calcium protein (CRT) expression in myoblast under cyclic tensile stress
stimulus.Methods:The culture-tensile stimulate model of myoblast in vitro was established. The strength group cells were cultured for 1
h, 6 h, 12 h, 24 h under 0.5 hz loading frequency and 10%tensile deformation amplitude. PCR and Western Blot technique was used to
detect the changes of CRT in the genetic level and protein level under cyclic tensile stress.Results:After 6 h cyclic tensile stress, the
expression of CRT mRNA and protein began to increase, after 12 h cyclic tensile stress, the expression of CRT mRNA and protein reached
a maximum (P＜0.01), the difference between 0 h group and 12 h has obvious statistical significance (P＜0.01).Conclusion:The
expression of CRT mRNA and protein can increase under continuous tensile stress stimulation. |
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