| 曹屹东 贾栋 衡立君 李维新.高频电刺激下丘脑后核对氟哌啶醇致大鼠运动不能
的影响[J].,2014,14(9):1633-1636 |
| 高频电刺激下丘脑后核对氟哌啶醇致大鼠运动不能
的影响 |
| The Effect of High Frequency Stimulation of Posterior HypothalamicNucleus in Haloperidol-induced Akinesia rats |
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| DOI： |
| 中文关键词: 帕金森病 下丘脑后核 高频电刺激 跑步机 |
| 英文关键词: Parkinson's disease Posterior hypothalamic nucleus High frequency stimulation Treadmill |
| 基金项目:国家自然科学基金面上项目（81171135） |
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| 中文摘要: |
| 目的：利用氟哌啶醇致僵直大鼠模拟帕金森病（PD）的运动不能，通过高频电刺激下丘脑后核(PH)，观察大鼠僵直和运动能
力的变化，从而探讨PH 在PD治疗中潜在的应用价值。方法：将成年雄性SD 大鼠随机分为PH 刺激组、假刺激组和对照组，对
PH 刺激组和假刺激组大鼠双侧PH 置入双极刺激电极，腹腔注射氟哌啶醇30 min 后，PH刺激组给予持续高频电刺激(130 Hz,60
us, 100 uA)，分别利用爬杆实验和跑步机实验评价大鼠僵直程度和运动能力。结果：腹腔注射氟哌啶醇1.0 mg/kg 后，①大鼠呈僵
直状态，其潜伏期为167.88± 17.88 s, 给予双侧PH 高频电刺激后潜伏期显著缩短至77.5± 21.27 s（P<0.01）。②跑步机试验显示大
鼠跑动速度和跑动距离显著下降，分别为5.78± 0.90 cm/s 和8.06± 4.35 m（P<0.01），给予双侧PH高频电刺激后显著提高跑动速
度和跑动距离，分别为12.72± 3.66 cm/s 和98.61± 96.75 m（P<0.01）。结论：腹腔注射氟哌啶醇可模拟帕金森病的僵直和运动不
能症状，双侧高频电刺激PH 可显著拮抗氟哌啶醇对大鼠僵直和运动不能的作用，提示PH 为DBS治疗帕金森病运动不能的有效
刺激靶点，为临床DBS 刺激PH 治疗PD 提供实验依据。 |
| 英文摘要: |
| Objective:To explore the potential target for deep brain stimulation (DBS) in the treatment of Parkinson's disease by
observing changes after bilateral high frequency stimulation of the posterior hypothalamic nucleus (PH) post injection of haloperidol.Methods:Adult male SD rats were randomly divided into three groups: haloperidol and PH stimulation group (HAL+Stim), haloperidol
group (HAL) and saline group (Saline). Bipolar stimulation electrodes were bilaterally implanted into PH in PH stimulation group. Rats
in all three groups were injected with haloperidol and then the degree of catalepsy and akinesia were evaluated by bar test and treadmill
test, respectively. In the stimulation group, PH was bilaterally given high frequency stimulation (130 Hz, 60 uS, 100 uA) during
behavioral tests.Results:Thirty minutes after injection of haloperidol (1.0 mg/kg), rats were catalepsy. The treadmill tests showed that
the velocity and distance were significantly reduction in haloperidol treatment rats. And bilateral PH high frequency stimulation
significantly reduced the degree of catalepsy as well as improved the velocity and distance in treadmill test. Thirty minutes after injection
of haloperidol (1.0 mg/kg), ① Rats were catalepsy with the latency of 167.88± 17.88 s. Bilateral PH high frequency stimulation (130 Hz,
60 us,100 uA) significantly reduced the latency to 77.5± 21.27 s（P<0.01）. ②The treadmill tests showed that the velocity and distance
were significantly reduced, at 5.78± 0.90 cm/s and 8.06± 4.35 m respectively（P<0.01）. And bilateral PH high frequency stimulation
significantly improved the velocity and distance in treadmill test to 12.72 ± 3.66 cm/s and 98.61 ± 96.75 m, respectively（P<0.01）Conclusion:Injection of haloperidol mimics the catalepsy and akinesia symptoms of Parkinson's disease. High frequency stimulation of
PH can significantly reduce haloperidol-induced catalepsy and improve locomotion, suggesting that PH is a potential stimulation target
for DBS in the treatment of PD which provides the experimental basis for treatment PD by PH-DBS ultimately. |
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