| 汪云1 汪佳欣1 李红霞1 梅夏齐1 王媛媛1 宋波1 梁琦2△.聚乙二醇干扰素琢-2a 对慢性乙型肝炎患者树突状细胞
B7-H1 表达的影响*[J].,2014,14(8):1485-1489 |
| 聚乙二醇干扰素琢-2a 对慢性乙型肝炎患者树突状细胞
B7-H1 表达的影响* |
| Dynamic Changes of the Expression of B7-H1 on DCs in Chronic Hepatitis BPatients Treated with PEG-IFN alpha-2a* |
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| DOI： |
| 中文关键词: 聚乙二醇干扰素琢-2a 慢性乙型肝炎 树突状细胞功能 B7-H1 HBV-DNA |
| 英文关键词: PEG-IFN alpha-2a Chronic hepatitis B Dendritic cells (DCs) B7-H1 HBV-DNA |
| 基金项目:黑龙江省自然科学基金项目( D201076)；黑龙江省教育厅基金项目(11551250） |
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| 中文摘要: |
| 摘要目的:观察聚乙二醇干扰素α-2a 对慢性乙型肝炎患者外周血树突状细胞功能及B7-H1 的影响，探讨慢性乙型肝炎病毒逃
逸的的机制。方法：慢性乙型肝炎患者31例，给予聚乙二醇干扰素琢-2a180 μg 抗病毒治疗52周，分别于0、12、26、52周检测肝功
能、HBV-DNA；流式细胞术检测外周血mDC表面HLA-DR、CD80、CD86、CD83、CD1a、B7-H1 水平。根据患者HBV-DNA 水平，
将患者分为应答组（A 组）、非应答组（B 组），10 例健康志愿者作正常对照组（C 组）。结果：慢性乙肝患者的树突状细胞膜表面分
子HLA-DR、CD80、CD86、CD83、CD1a 的表达均降低。聚乙二醇干扰素α-2a 治疗后应答组膜表面分子HLA-DR、CD80、CD86、
CD83、CD1a 的表达高于非应答组65.3± 6.2 %vs 44.2± 5.5 %,67.2± 7.4%vs 37.3± 7.2 %,68.4± 3.6 %vs 42.5± 7.3 %，65.6± 6.8
%vs 43.2± 3.9 %, 49.4± 9.5 %vs 37.5± 7.9 %，(P <0.05)。应答组B7-H1 表达水平较治疗前下降，非应答组B7-H1 水平无明显变
化12.73± 3.8%vs 25.24± 2.92 %，(P<0.05)。结论：慢性乙型肝炎患者树突状细胞功能低下，聚乙二醇干扰素α-2a 治疗可以提高
树突状细胞功能，降低B7-H1 表达，促进HBV-DNA 的清除。树突状细胞功能低下及B7-H1 高表达是乙型肝炎病毒免疫逃逸的
因素之一。 |
| 英文摘要: |
| ABSTRACT Objective:To study the dynamic changes of the expression of B7-H1 on dendritic cells (DCs) in chronic hepatitis B
(CHB) patients undergoing PEG-IFN alpha-2a therapy. Methods:31 patients with chronic hepatitis B were given with PEG-IFN alpha-2a
180 μg, once a week for 52 weeks. Hepatic function and HBV-DNA were detected at week of 0, 12, 26, 52 respectively. The expression
of HLA-DR, CD80, CD86, CD83, CD1a, B7-H1 on DCs were detected by flow cytometry. According to HBV-DNA levels, the patients
were divided into responding group (A), non-responding group (group B), 10 healthy volunteers as control group (group C).Results: The
expression of HLA-DR, CD80, CD86, CD83, CD1a on DCs in patients with CHB were lower than control group (P<0.05). After
PEG-IFN alpha-2a therapy, the expression of HLA-DR, CD80, CD86, CD83, CD1a on DCs upregulated in responding group than
non-responding group (65.3± 6.2%vs 44.2± 5.5 %, 67.2± 7.4%vs 37.3± 7.2 %, 68.4± 3.6%vs 42.5± 7.3 %, 65.6± 6.8%vs 43.2±
3.9 %, 49.4± 9.5 % vs 37.5± 7.9 %, (P<0.05)). B7-H1 expression on DCs were persistently decreased in the responding group after
PEGIFN 琢-2a treatment, while nonresponding group maintained high level of B7-H1 expression (12.73± 3.8%vs 25.24± 2.92 %,(P <0.
05)). Conclusion:PEG-IFN α-2a therapy can improve the function of dendritic cells. The expression of B7-H1 on DCs was
down-regulated with HBV-DNA clearance after PEGIFN α-2a treatment. Dendritic cell dysfunction indicated by the increased B7-H1
expression is one of the factors of hepatitis B virus immune escape. |
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