| 彭小娟1 白海2△ 王存邦2 葸瑞2 晁二涛1.黄芪甲苷对人骨髓间充质干细胞体外增殖
及细胞因子表达的影响*[J].,2014,14(8):1452-1455 |
| 黄芪甲苷对人骨髓间充质干细胞体外增殖
及细胞因子表达的影响* |
| Effect of Astragaloside IV on the Proliferation and Cell FactorExpression in hBMSC* |
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| DOI： |
| 中文关键词: 黄芪甲苷 骨髓间充质干细胞 增殖 细胞因子 |
| 英文关键词: Astragaloside IV Bone marrow mesenchymal stemcells Proliferation Cell factor |
| 基金项目:甘肃省科技重大专项（1102FKDA005） |
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| 中文摘要: |
| 摘要目的：探讨黄芪甲苷对人骨髓间充质干细胞体外增殖及细胞因子表达的影响。方法：采用Percoll 密度离心法和贴壁法分离
纯化hBMSC；流式细胞术检测hBMSC 表面标志；MTT 法检测不同浓度黄芪甲苷干预72 h后hBMSC 的增殖情况；Real-time
PCR 法检测经黄芪甲苷干预后hBMSC对SCF、VEGF、SDF-1、GM-CSF mRNA 的表达水平。结果：成功分离培养出hBMSC；不同
浓度（20、40、80、160、320 mg / mL）的黄芪甲苷可促进hBMSC增殖(P<0.05)，其中160 mg/mL 组促增殖最明显；黄芪甲苷可促进
hBMSC对SCF、VEGF、SDF-1 mRNA的表达，而GM-CSF mRNA 表达无明显变化。结论：黄芪甲苷可促进hBMSC 体外增殖，可
能与其促进hBMSC 对SCF、VEGF、SDF-1 mRNA表达有关。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the effect of Astragaloside IV on the proliferation and the expression of cell factor in human
bone marrow mesenchymal stem cells (hBMSC).Methods: Human bone marrow mesenchymal stem cells were isolated from bone marrow
by percoll isodensity centrifugation and adherent method. The surface marker of hBMSC were detected by flow cytometer. The proliferation
of hBMSC with different concentration Astragaloside IV for 72 hours were evaluated by MTT. The expressions of SCF, VEGF,
SDF-1, GM-CSF mRNA were measured by Real-time PCR.Results: The hBMSC were isolated successfully. Astragaloside IV at 20, 40,
80, 160, 320 mg/mL significantly increased the proliferation of hBMSC (P<0.05), especially the group of 160 mg/mL had the most obvious
effect. Astragaloside IV could stimulated the expressions of SCF, VEGF, SDF-1 mRNA, but GM-CSF mRNA were not change significantly.
Conclusiong:Astragaloside IV could increase the proliferation of hBMSC and improve the expressions of the SCFV, EGF,
SDF-1 mRNA. |
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