| ABSTRACT Objective:To investigate the primary cultured rabbit nucleus pulposus cells transfected with adenovirus vector
mediated human bone morphogenetic protein-7 effect on its biological activity after (hBMP-7) gene.Methods: Depending on nucleus
pulposus cells transfected way, into hBMP7 group, LacZ group, untransfected three groups, hBMP7 group using adenoviral
vector-mediated transfection hBMP7, LacZ transfected with LacZ gene, not transfected genes without any transfection. Comparison of
the three groups of cell proliferation, sulfate glycosaminoglycan ((GAG) production , whether the difference in type Ⅱ collagen
production. Results:After treatment and transfection time on cell proliferation, GAG production, type Ⅱ collagen production had
interaction (P<0.05), hBMP7 cell proliferation, GAG production, type Ⅱ collagen production is higher than LacZ group, the
non-transfected group, the difference was statistically significant (P<0.05), cell proliferation LacZ group and untransfected group, GAG
production , Ⅱ collagen production was no significant difference (P>0.05).Conclusion: hBMP-7 gene transfection can increase the
proliferation of nucleus pulposus cells and promote their produce extracellular matrix. |
