| 刘宣1 王海嵘1 刘佳福1 陈向军2 卢孔渺1 潘曙明1△.白藜芦醇对氧糖剥夺/ 再灌注损伤的PC12细胞的保护作用
及其作用机制*[J].,2014,14(8):1423-1427 |
| 白藜芦醇对氧糖剥夺/ 再灌注损伤的PC12细胞的保护作用
及其作用机制* |
| Protective Effect of Resveratrol on the Injury of PC12 Cells Induced byOGD/R and Its Mechanisms* |
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| DOI： |
| 中文关键词: 白藜芦醇 氧糖剥夺/再灌注 细胞凋亡 ROS 生成 SIRT1 |
| 英文关键词: Resveratrol OGD/R Apoptosis ROS generation SIRT1 |
| 基金项目:上海市公共卫生人才培养计划（GWDTR201219） |
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| 中文摘要: |
| 摘要目的：探讨白藜芦醇对氧糖剥夺/再灌注(OGD/R)损伤的PC12 细胞的保护作用及其机制。方法：体外培养PC12 细胞，分为
对照组，白藜芦醇组，OGD/R 组及OGD/R+ 白藜芦醇组。以改良的噻唑蓝法测定细胞活性，采用Annexin V-FITC/PI 双染法检测
细胞的凋亡率，用双氢罗丹明（DHR）检测细胞内活性氧簇（ROS）的水平，采用蛋白印迹法(western blot)分析SIRT1 的蛋白表达情
况。结果：与对照组相比，经过OGD/R 损伤后，细胞活力显著降低。而在OGD/R 的同时给予10 μmol/L的白藜芦醇处理，可以明
显提高细胞活力。流式细胞仪检测发现，10 μmol/L 的白藜芦醇可以显著地减少OGD/R 引起的细胞凋亡，抑制细胞内的ROS 产
生。western blot 的结果提示，与对照组比较，白藜芦醇可提高SIRT1 的蛋白表达水平。结论：白藜芦醇可以通过抑制ROS 的产生
和上调SIRT1的表达等机制而发挥其对抗氧糖剥夺/再灌注损伤的神经保护性作用。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the protective effect of resveratrol (RSV) on the injury of PC12 cells induced by oxygen
glucose deprivation /reperfusion (OGD/R) and its mechanisms.Methods: PC12 cells cultured in vitro were divided into control group,
RSV group, OGD/R group and OGD/R+RSV group. The cell viability was assessed by the modified MTT method. Annexin V-FITC/PI
double staining was used to detect apoptosis rate. DHR123 was used to measure cellular ROS levels. Western blot was used to study the
expression level of SIRT1.Results: Compared to the normal control group, the cell viability decreased significantly after OGD/R and
giving 10 μmol/L RSV during OGD/R could significantly increase cell viability. Based on the results of flow cytometry, we found 10
μmol/L RSV markedly inhibited OGD /R-induced apoptosis and reduced intracellular ROS generation. Result of Western blot showed
that RSV could increase the expression of SIRT1 compared with control group. Conclusion:RSV was found to have a neuroprotective
effect against OGD/R-induced injury by attenuating ROS production and up-regulating the expression level of SIRT1. |
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