| 周桂英
1
李志燕
2
杨春兰
2
苏 慧
2
李振泽
2
张薇薇
2
曾 亮
2,3 △.细胞衰老和凋亡相关蛋白表达在宫颈鳞癌变中的意义[J].,2014,14(6):1065-1068 |
| 细胞衰老和凋亡相关蛋白表达在宫颈鳞癌变中的意义 |
| Significance of Senescence and Apoptosis Associated Proteins Expression inCervical Squamous Carcinogenesis |
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| DOI： |
| 中文关键词: 细胞衰老 细胞凋亡 宫颈鳞癌 |
| 英文关键词: Cell senescence apoptosis squamous carcinoma of the cervix |
| 基金项目:湖南省科学技术厅科技计划项目 （2010FJ3154 ） |
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| 中文摘要: |
| 摘要 目的：许多细胞周期调控因子和衰老相关标志物如 p14ARF、 p15INK4b、 p16INK4a 和 p53 在 G1 细胞周期阻滞和癌基因诱
导的衰老中意义重大。这些关键的调节蛋白在多种恶性肿瘤中经常发生突变或是缺失。在本研究中将探讨这些因子在宫颈癌发
生中的意义。 方法： 在本研究中在正常宫颈上皮、 宫颈上皮内瘤变和宫颈鳞癌中， 应用免疫组织化学方法检测 p14ARF、 p15INK4b、
p16INK4a、 Bcl-2、 p53 表达， 并分析它们的表达与宫颈癌变的相关性。结果： p16INK4a 在正常宫颈鳞状上皮 10% （ 2/20 ） 表达阴性，
在大部分 CIN 和宫颈鳞癌中表达阳性， 其中在 85% （ 17/20 ） CIN 和 75% （ 15/20 ） 鳞癌中呈弥漫性强阳性表达， CIN 和宫颈鳞癌中的
阳性表达率显著高于正常上皮 （P<0.01 ）， CIN 和宫颈鳞癌的间表达率无显著差异。 p15INK4b 在正常宫颈鳞状上皮中 65% （13/20 ）
表达弱阳性，在 100% (20/20 ） CIN 和 95% （ 19/20 ） 宫颈鳞癌中表达弥漫性阳性，各组之间阳性表达率无显著性差异（ P>0.05 ）。
p14ARF 在 40% （ 8/20)正常宫颈上皮细胞中表达呈弱阳性(1+ ） ，在宫颈鳞癌中表达呈弥漫性强阳性 90%(18/20 ） ，在 45% （ 9/20 ） CIN
中表达阳性， 各组之间阳性表达率无显著性差异（ P>0.05 ）。Bcl-2 在 20% （ 4/20 ） 正常宫颈上皮表达呈弱阳性，在 18/20CIN 中其表
达强度和比率均增加， 阳性表达率为 90% （18/20 ）， Bcl-2 在鳞癌中 700% （14/20 ） 呈强阳性和弥漫阳性， CIN 和宫颈鳞癌中的阳性
表达率显著高于正常上皮 （ P<0.01 ）， CIN 和宫颈鳞癌的间表达率无显著差异。P53 免疫组化染色显示在正常宫颈上皮为表达为
20% （4/20)，在大多数 CIN25% (5/20)和鳞癌中核阳性 85% (17/20)，在鳞癌中的阳性表达率显著高于正常宫颈上皮和 CIN 病变
(P<0.05)。结论： 宫颈鳞癌变涉及包括细胞凋亡和细胞衰老在内的多种信号分子表达异常， 这些分子可能在宫颈鳞癌发生发挥重
要作用并在宫颈癌早期诊断中有重要意义。 |
| 英文摘要: |
| ABSTRACT Objective：Some cell cycle regulators and Senescence associated proteins, such as p14ARF, p15INK4b, p16INK4a,
p53 are important in G1 cell cycle arrest and oncogene-induced senescence. The genes of these key regulator proteins are often mutated
or deleted in various malignancies. This study was to explore the significance of these factors in the pathogenesis of cervical carcinoma.
Methods：Positivity rate of P16INK4a in normal cervical squamous epithelium was 10% (2/20), and 85% (17/20) CIN and 75% (15/20) in
squamous cell carcinoma which showed diffuse strong positive expression. The positivity rate of P16INK4a in CIN and cervical squamous cell carcinoma was significantly higher than that of normal epithelium (P<0.01), and there was no significant difference between
CIN and squamous cell carcinoma of the cervix. Positivity rate of P15INK4b in normal cervical squamous epithelium was 65% (13/20),
and 100% (20/20) in CIN and 95% (19/20) in cervical squamous cell carcinoma with diffuse positive expression, there was no significant
difference of the positive expression rate between the groups (P>0.05). Positivity rate of P14ARF (8/20) was 40% in normal cervical epithelial cells, and 90% (18/20) in cervical squamous cell carcinoma with diffuse strong positive expression, 45% (9/20) expression of
CIN, and no significant difference of the positive expression rate between the groups (P>0.05). Positivity rate of Bcl-2 was 20% (4/20) in
normal cervical epithelium, and 90% (18/20) in CIN, 70% (14/20) in squamous cell carcinoma with strongly positive and diffuse staining, the positive rate of CIN and cervical squamous cell carcinoma was significantly higher than that in normal epithelium (P<0.01), and
there was no significant difference between CIN and cervical squamous cell carcinoma. P53 immunohistochemical staining showed that
in normal cervical epithelial expression was 20% (4/20), 25% (5/20) in CIN and 85% (17/20) in squamous cell carcinoma, the positive
rate of CIN and cervical squamous cell carcinoma was significantly higher than that in normal epithelium (P<0.05).Conclusion: Squa-mous cell carcinoma of the uterine cervix was related to abnormal expression of many signal molecules including cell apoptosis and cell
senescence, and playeda significant role in cervical squamous cell carcinogenesis and early diagnosis for cervical carcinoma. |
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