| ABSTRACT Objective:To explore the mechanism of Xuebijing treatment by observing the effect on inflammatory cytokine in rat
with chronic obstructive pulmonary disease (COPD) after Xuebijing dealing.Methods: Tirty-six Wister rats were divided intoa control
group,a COPD group and a dealing group at random. The COPD rat model was established by intratracheal instillation of
lipopolysaccharide (LPS) twice and exposuring to cigarette smoke daily for 30 days, and the dealing group was given Xuebijing injection
for dose of 4 mL/Kg weight every day in last 15 days. The spirometry was conducted and the pathological changes were observed after
the model was established. The concentration of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8) in serum and
bronchoalveolar lavage fluid was detected by enzyme-linked immunosorbent assay.Results: Significant decrease of FEV0.3/FVC[(57.8±
5.6) %] and dynamic compliance of lung (Cdyn) [(0.098 ± 0.006) cmH2 O · mL
-1
· s
-1
] , and increase of airway resistance (RI) [(0.802 ±
0.070) mL/cmH2 O] were found in the COPD group compared with the control group [(81.4± 3.1) %, (0.195± 0.012) cmH2 O · mL
-1
· s
-1
,
(0.224 ± 0.069) mL/cmH2O] (t=-13.940, -15.165, -22.389,P＜ 0.01). Significant increase of FEV0.3/FVC [(67.2 ± 3.2) %] and dynamic
compliance of lung (Cdyn) [(0.142± 0.024) cmH2 O · mL · s
-1
], and decrease of airway resistance (RI) [(0.475± 0.050) mL/cmH2O] were
found in the dealing group compared with the COPD group (t=-5.552, -6.927, -12.658,P＜ 0.01). Conclusion:The concentration of TNF-α, IL-6, IL-8
[(27.8 ± 7.4) pg/mL, (340.0 ± 79.6) pg/mL, (209.9 ± 82.1) pg/mL, (337.3 ± 96.4) pg/mL, (37.7 ± 11.4) pg/mL, (69.6 ± 18.9) pg/mL]in
serum and bronchoalveolar lavage fluid of the COPD rats were found to significantly increase compared with the control rats(t=-12.466,
-19.648, -11.749, -16.364, 12.550, 14.834,P＜ 0.01), while the concentration of TNF-α, IL-6, IL-8 [(9.6± 5. 6) pg/mL, (45.6± 22.9) pg/mL,(36.3± 17.9) pg/mL, (42.9± 20.5) pg/mL, (10.3± 5.6) pg/mL, (15.7± 8.0)pg/mL] in the dealing rats were found to singificantly decrease
compared with the COPD rats (t=-9.367, -17.390, -10.106, -14.631, 10.475, 12.772,P＜ 0.01). The concentration of TNF-α,
IL-6, IL-8 in serum and bronchoalveolar lavage fluid of the COPD rats were found to significantly increase, which can significantly
decrease by Xuebijing treatment. Those changes confirmed with their clinical symptoms, lung function and pathological changes. So we
speculated that Xuebijing may downgrade expression of key proinflammatory cytokines in COPD inflammatory, including TNF-α, IL-6
and IL-8, further inhibit the cascade effect of airway inflammation, by which it played strong anti-inflammatory effects. |
