| ABSTRACT Objective：To investigate the effect of edaravone on malonyldialdehyde (MDA), myeloperoxidase (MPO), renal tissue
superoxide dismutase (SOD), inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS) after canine autologous renal transplantation. Methods:18 weight matched healthy mongrel dogs were randomly divided into sham operation group (group S),
edaravone group (group ED) and normal saline group (group PS), with 6 cases in each group. Group S: only kidney operation resection.
Group ED: before clamping in the donor intravenous edaravone 10mg/kg, UW lavage and then use the 200 mL to join the edaravone
10mg/kg donor kidney and the preservation of donor kidney preservation solution for 8 hours in the same, and at the beginning of reperfusion immediately given edaravone 10 mg/kg in receptor intravenous. Group PS:with the same method using the same volume of saline.
We detected the activities of MDA, MPO, SOD, iNOS and eNOS after reperfusion for 4H, and postoperative 24h detection of serum creatinine (Cr), blood urea nitrogen (BUN) concentration, while pathological changes of renal tissue in each group were observed under light
microscope.Results: In PS group, MDA was significantly higher than those in S group and ED group (P<0. 05), iNOS of PS group were also
lower than those inS group and ED group (P<0.05). In group PS, SOD, eNOS content was significantly lower than those in S group and
ED group (P<0.05), iNOS of PS group was higher than those in S group and ED group (P<0.05), the pathological injury in ED group was
significantly reduced.Conclusion： Edaravone can reduce ischemia reperfusion injury in renal transplantation, and its mechanism may be
related to reduce renal lipid peroxidation. |
