郭予和 何 颖 邹泽红 刘雪婷 许晨霞 陶爱林
△.扁豆过敏原 Len c3 蛋白 B 细胞抗原表位预测及同源建模 *[J].,2014,14(6):1017-1021 |
扁豆过敏原 Len c3 蛋白 B 细胞抗原表位预测及同源建模 * |
Prediction of the B Cell Antigenic Epitopes and Homology Modeling Studyof Lentil Allergen Len c 3* |
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DOI: |
中文关键词: 扁豆过敏原 Lenc3 结构 抗原表位 |
英文关键词: Lentil allergen Lenc 3 Structure Antigenic epitopes |
基金项目:国家重大科技专项重大课题 (2011ZX08011-005 ) ; 国家自然科学基金项目( 30771240 ) |
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中文摘要: |
摘要 目的: 通过应用生物信息学软件拟、分析预测扁豆过敏原 Lenc3 蛋白的结构、 性质及 B 细胞抗原表位, 为探索基于扁豆
过敏原 Lenc3 抗原表位的改造提供依据。方法: 从 Uniprot 蛋白质数据库中得到扁豆过敏原 Len c3 的氨基酸序列, 通过生物信
息学软件 Swiss-Model 及 Swiss-PdbViewer 4.0 拟和分析扁豆过敏原 Lenc3 蛋白的结构,使用 DNAStar 软件对其 B 细胞抗原
表位进行拟、分析预测。结果: 扁豆过敏原 Lenc3 蛋白为疏水性蛋白,在氨基酸残基第 7-26 位有一跨膜区, Ramachandran 图评
估扁豆过敏原 Lenc3 蛋白的三维结构显示其空间构象稳定, Lenc3 蛋白潜在的 B 细胞抗原表位为 35-36, 48-50, 66-71, 87-90。 结
论: 本研究通过对扁豆过敏原 Lenc3 蛋白进行生物信息学分析获得了该过敏原的结构、 性质及潜在的 B 细胞抗原表位, 为进一
步了解和掌握扁豆过敏原 Len c3 蛋白的结构功能以及抗原性改造、单克隆抗体制备、 表位疫苗设计等提供重要的线索。 |
英文摘要: |
ABSTRACT Objective:To predict and analyze the structure, biological characteristics and B cell antigenic epitopes of lentil allergen Len c 3 by using bioinformatics software, so as to provide clues for modifying the antigenic epitopes of lentil allergen Lenc 3.
Methods:The amino sequence of lentil allergen Len c 3 was acquired from Uniprot protein database. The structure of lentil allergen Len c 3 was
simulated and analyzed by the bioinformatics software Swiss-Model and Swiss-PdbViewer 4.0, and the B cell antigenic epitopes of lentil
allergen Len c 3 were predicted and analyzed by using DNAStar.Results: Lentil allergen Lenc3 with a transmembrane domain belongs
to a hydrophobic protein. The Ramachandran plot showed the spatial conformation of Lentil allergen Len c 3 was stable. The potentialB
cell antigenic epitopes of Len c 3 were at the region of 35-36, 48-50, 66-71, 87-90.Conclusion: This study predicted and analyzed the
structure, biological characteristics and potential B cell antigenic epitopes of lentil allergen Lenc 3, which provided important clues for
further understanding and mastering the structure and biological characteristics even antigen modification, monoclonal antibody preparation and epitope vaccine design of Lentil allergen Lenc 3. |
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