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摘要目的：观察钛颗粒对小鼠颅骨中OPG/RANKL mRNA及其蛋白表达的影响，探讨关节置换术后骨溶解的发生机制。方法：取成年BALB/C 小鼠40 只，随机分为假手术组、钛颗粒低剂量组、钛颗粒中剂量组及高剂量组，每组10 只。除假手术组外，其余各组分别将钛颗粒15、30、60 mg涂抹于小鼠颅骨表面后缝合切口。8 周后取颅骨组织及外周血，运用real-time PCR 及ELISA 技术测定OPG/RANKL基因及蛋白表达情况。结果：与假手术组相比，钛颗粒低剂量组外周血中OPG蛋白表达及颅骨组织中OPG mRNA 的表达均显著上升(P<0.01)，外周血中RANKL蛋白表达降低，但无统计学差异，颅骨组织中RANKL mRNA 表达无显著差异；中剂量组及高剂量组外周血中OPG 蛋白表达显著降低(p<0.01)，RANKL蛋白表达显著升高(P<0.01)，OPG mRNA 表达显著降低(P<0.01)，RANKL mRNA 表达显著升高(P<0.01)。低中高三种不同剂量钛颗粒组组间相比，外周血中OPG、RAKNL蛋白及颅骨组织中其mRNA表达均存在明显差异（P<0.01），高剂量组对OPG、RANKL蛋白及mRNA 表达的影响更显著。结论：钛颗粒可以改变OPG/RANKL的mRNA 及蛋白表达量，这可能是其导致关节置换术后体骨溶解进而产生松动的原因之一。

英文摘要:

ABSTRACT Objective:To study the influence of titaniumparticles on the mouse skull OPG/RANKL mRNA and protein expression, and explore the osteolysis mechanisms after joint replacement surgery. Methods:40 adult BALB/C mouse were randomly divided into the sham operation group, low-dose titanium particles group, middle dose group and high dose group,(10 mice in each group). In addition to the sham group, the rest groups were smeared titanium particles 15 mg, 30 mg, 60 mg on the surface of the skull, then suture incision. 8 weeks later, the skull tissue and peripheral blood were taken. Real-time PCR and ELISA techniques were used to determine the OPG/RANKL mRNA and protein expression. Results:Compared with the sham group, OPG protein expression in the peripheral blood and OPG mRNA expression in skull tissues of low dose titanium particles group were significantly increased (P<0.01), RANKL protein expression in peripheral blood decreased, but no significant difference was found. RANKL mRNA expression in skull tissues was also had no significant difference. OPG protein expression in the peripheral blood of middle dose group and high dose group were significantly decreased (P<0.01), RANKL OPG protein expression in the peripheral blood were significantly increased (P<0.01), OPG mRNA expression were significantly decreased(P<0.01), RANKL mRNA expression were significantly increased(P<0.01). OPG/RAKNL protein expression in peripheral blood and mRNA expression in skull tissue were significantly different (P<0.01), the effect of high dose group on the expression of OPG/RANKL protein and mRNA was more significant.Conclusion: Titanium particles could change the OPG/RANKL mRNA and protein expression, which may be one of the cause of osteolysis after joint replacement surgery.

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