| 魏燕熊佳易王蔼明赵军王宁张兰梅△.Genistein 对卵巢癌铂类耐药细胞株CP70 增殖凋亡的影响
及与细胞内ROS 的相关性[J].,2014,14(5):841-845 |
| Genistein 对卵巢癌铂类耐药细胞株CP70 增殖凋亡的影响
及与细胞内ROS 的相关性 |
| Effects of Genistein on Proliferation, Apoptosis and the Level of ROSin Human Ovarian Carcinoma Cell CP70 |
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| DOI： |
| 中文关键词: Genistein 活性氧 卵巢癌 细胞凋亡 NAC |
| 英文关键词: Genistein Reactive oxygen species Ovarian carcinoma Apoptosis NAC |
| 基金项目: |
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| 中文摘要: |
| 摘要目的：探讨Genistein 对卵巢癌铂类耐药细胞CP70 增殖、凋亡的影响及与细胞内活性氧水平的关系。方法：采用MTT 法检
测Genistein 对CP70 细胞增殖的影响；流式细胞仪分析不同药物处理后对细胞凋亡的影响，线粒体膜电位及细胞内ROS水平的
变化情况。结果：Genistein 对CP70 细胞增殖表现出剂量和时间依赖性的抑制作用，并能诱导其凋亡；Genistein 作用于CP70 细胞
后，可使其线粒体膜电位降低，并引发了细胞内ROS 水平的显著升高；ROS抑制剂NAC 预处理CP70 细胞后，有效抑制了ROS
的产生，并降低了细胞凋亡率，与未加NAC组相比差异有显著性（P<0.05）。结论：Genistein能抑制铂类耐药卵巢癌细胞CP70 的
增殖，并促进其凋亡，这与细胞内ROS 水平的升高有关，可能是Genistein 抗肿瘤诱导细胞凋亡的机制之一。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the effects of Genistein on proliferation, apoptosis and the level of ROS in drug resistant
Human Ovarian Carcinoma Cell CP70. Methods:The growth inhibitory effect of different drugs on CP70 cells was detected by MTT
assay. The apoptosis rate was determined by flow cytometry. Mitochondrial membrane potential and the level of ROS were measured by
flow cytometry.Results: The exerted inhibitory effect of Genistein on CP70 cells in a time and dose dependent manner, and it could
induce the apoptosis of CP70 cells. Genistein reduced the mitochondrial membrane potential and increased the level of ROS. After
pretreatment with antioxidant NAC, the level of ROS was effectively inhibited, meanwhile mitochondrial membrance potential increased
and apoptotic rate decreased.Conclusion: Genistein inhibited the proliferation and induced apoptosis, and is related to the increased of
ROS in cisplatin-resistant ovarian cancer cell CP70. It may be one of the mechanismof apoptosis induced by Genistein in CP70 cells. |
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