| 刘彦陈绍良△ 徐祖玲刘志忠阚静毛文星.大鼠主动脉内皮细胞的分离培养和鉴定[J].,2014,14(5):834-836 |
| 大鼠主动脉内皮细胞的分离培养和鉴定 |
| Isolation Culture and Identification of Rat Aortic Endothelial Cells |
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| DOI： |
| 中文关键词: 大鼠 主动脉内皮细胞 培养 鉴定 复苏 |
| 英文关键词: Rat Aortic endothelial cells Culture Identify Recovery |
| 基金项目: |
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| 中文摘要: |
| 摘要目的：探索大鼠主动脉原代内皮细胞体外培养方法，为体外研究提供细胞模型。方法：分离大鼠主动脉，直接贴壁于培养皿
中，荧光倒置显微镜观察细胞形态，免疫组化Ⅷ因子相关抗原染色鉴定细胞。结果：约24 小时组织块边缘有游离的新生细胞长
出，7天即融合成片。消化传代后细胞呈短梭形或三角形，单层生长，铺路石状，Ⅷ因子表达阳性，呈指数增殖。冻存后复苏细胞活
性均超过90%。结论：用贴壁法成功建立了大鼠血管内皮细胞体外培养方法，冻存细胞存活率高，为体外研究提供了稳定的模型。 |
| 英文摘要: |
| ABSTRACT Objective：To investigate the culture methods of primary rat aortic endothelial cells in vitro.Methods： Rat aorta was
isolated, directly attached to the wall in a petri dish, and inverted microscope cell morphology. Immunohistochemistry factor VIII-related
antigen was used to stain cells. Results：Organization block edge free new cells grow, after approximately 24, and integrated into the film
7d later. Cells were subcultured short spindle-shaped or triangular, monolayer, cobblestone, positive expression of factor VIII, the
exponential proliferation. Recovery was more than 90% cell viability after cryopreservation. Conclusion：Vascular endothelial cells in
vitro method was successfully established by adherent, and the survival rate of frozen cells was high, which providing a stable model for
in vitro studies. |
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