| 徐志红1 张悦民1 郁佳敏2 高蓓莉2 胡家安1△.细胞自噬在吉非替尼治疗非小细胞肺癌中的作用
及其机制研究[J].,2014,14(5):822-825 |
| 细胞自噬在吉非替尼治疗非小细胞肺癌中的作用
及其机制研究 |
| The Role of Geftinat in Non Small Cell Lung Cancer Induced by Autophagy |
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| DOI： |
| 中文关键词: 吉非替尼 非小细胞肺癌 细胞自噬 |
| 英文关键词: Geftinat Non small cell lung cancer Autophagy |
| 基金项目: |
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| 摘要点击次数: 860 |
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| 中文摘要: |
| 摘要目的：观察吉非替尼对非小细胞肺癌细胞自噬的影响，并探讨其机制。方法：将BalB/CA-nu 品系裸小鼠分成两组，各组20
只，均造肺癌模型，其中吉非替尼治疗组小鼠在肺癌组模型的基础上给予吉非替尼25mg/kg 14d，停药后剖杀。取组织切片，通过
免疫荧光、RT-PCR以及Western blot 的试验方法检测自噬相关基因Beclin1 和MAPLC3 的表达。结果：免疫荧光、RT-PCR以及
Western blot 的试验方法检测均发现肺癌组小鼠组织中Beclin1 和MAPLC3 表达较低，而在吉非替尼处理组中，Beclin1 和
MAPLC3 的表达明显升高，差异有统计学意义。结论：吉非替尼可以通过增强细胞自噬从而发挥对非小细胞肺癌的抑制作用。 |
| 英文摘要: |
| ABSTRACT Objective:To observe The role of Geftinat in non small cell lung cancer induced by autophagy and to investigate the
mechanism. Methods:40 nude mice were randomly divided into two groups, the group with geftinat was treated by infusing the stomach
with the medicine, 25mg/kg. The expression of Beclin1 and MAPLC3 were determined by immunofluorescence, RT-PCR and Western
blot. Results:The Mrna and protein level of Beclin1 and MAPLC3 in the group with geftinat treating were both significant higher by
immunofluorescence, RT-PCR and Western blot.Conclusion: Geftinat can inhibit the progress of non small cell lung cancer by influence
autophagy. |
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