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摘要目的：探讨增殖诱导配体APRIL，X 染色体连锁凋亡抑制蛋白XIAP 及半胱氨酸天冬氨酸蛋白酶caspase-9 在人肝癌组织中的表达及意义。方法：采用实时荧光定量逆转录-聚合酶链反应（reverse transcription-polymerase chain reaction, RT-PCR）方法，分别检测62 例肝癌患者的肝癌组织、癌旁组织及30例正常人的肝脏组织中APRIL, XIAP 和caspase-9 的表达。对比分析APRIL, XIAP 和caspase-9 的表达与肝癌临床病理参数的关系。结果：在肝癌组织中，APRIL和XIAP 的表达明显高于其在癌旁组织及正常肝脏组织中的表达(P<0.05)；caspase-9 的表达则明显低于癌旁组织以及正常肝脏组织(P<0.05)；在癌旁组织和正常肝脏组织中， APRIL, XIAP和caspase-9 的表达无显著差异(P>0.05)。APRIL和XIAP 在肝癌组织中的表达与甲胎蛋白、肿瘤组织学分级、临床病理TNM分级有关(P<0.05)。结论：APRIL, XIAP和caspase-9 在肝癌组织中的表达与肝癌临床病理特征密切相关，不仅是检测肝癌的重要生物学标志，也是潜在的肝癌治疗靶点。

英文摘要:

ABSTRACT Objective:To investigate the expression and significance of APRIL, XIAP and caspase-9 in hepatocellular carcinoma (HCC).Methods: The reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression levels of APRIL, XIAP and caspase-9 in HCC. The liver cancer and its adjacent tissues of 62 patients with hepatocellular carcinoma, and the normal tissues of 30 healthy people were detected respectively. The correlations of APRIL, XIAP and caspase-9 expressions with the clinicopathological features were compared and analyzed. Results:In the liver cancer tissues, the expression levels of APRIL and XIAP were significantly higher than those of the adjacent tissues and the normal tissues (P<0.05), while the expression levels of caspase-9 was significantly lower than those of the adjacent tissues and the normal tissues (P<0.05); There was no statistically significant difference about the expression levels of APRIL, XIAP and caspase-9 between the adjacent tissues and the normal tissues (P>0.05). There is a close relationship among the expression levels of APRIL, XIAP, caspase-9 and the alpha fetoprotein level, the histological differentiation and the tumor node metastasis classification (P<0.05). Conclusion:The expressions of APRIL, XIAP and caspase-9 in HCC are closely relative to the clinicopathologic features of HCC which could be the potential markers for HCC and the target for the treatment of HCC.

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