| 王婷 阮林高 戈梅 钱秀萍.一种利用荧光标记检测氨肽酶N抑制剂与肿瘤细胞结合的方法[J].,2014,14(4):634-638 |
| 一种利用荧光标记检测氨肽酶N抑制剂与肿瘤细胞结合的方法 |
| A method for Detecting the Binding Activities of Aminopeptidase NInhibitors to Tumor Cell by Fluorescein |
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| DOI： |
| 中文关键词: 氨肽酶N 抑制剂 LYRM03 Bestatin 异硫氰酸荧光素 |
| 英文关键词: Aminopeptidase N inhibitors LYRM03 Bestatin Fluorescein isothiocyanate（FITC） |
| 基金项目:国家重大新药创制专项（2010zx09401-403) |
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| 中文摘要: |
| 目的：建立利用荧光标记法检测氨肽酶抑制剂和肿瘤细胞结合的方法。方法：以异硫氰酸荧光素（FITC）标记氨肽酶N抑制
剂LYRM03 和Bestatin，制备荧光探针FITC-LYRM03、FITC-Bestatin，应用荧光显微成像观察和流式细胞仪检测标记化合物
FITC-LYRM03、FITC-Bestatin对肿瘤细胞的结合与氨肽酶N 抑制活性的相关性。结果：化合物LYRM03 和Bestatin 具有肿瘤细
胞的氨肽酶N抑制活性，荧光标记化合物FITC-LYRM03、FITC-Bestatin 能与肿瘤细胞有不同程度的结合。结论：标记化合物
FITC-LYRM03、FITC-Bestatin和肿瘤细胞的结合与对肿瘤细胞的氨肽酶N抑制活性相一致。 |
| 英文摘要: |
| Objective:To build a fluorescein labeling method to detect the bingding of aminopeptidase N inhibitors with tumor
cells.Methods:AminopeptidaseN inhibitors LYRM03 and Bestatin were labeled with fluorescein isothiocyanate (FITC), and fluorescein
-labeled compounds of FITC-LYRM03 and FITC-Bestatin were purified. The relationship between binding activities to tumor cells and
inhibitory activities of aminopeptidase N inhibitors was detected by using fluorescence microscope and flow cytometer. Results:Compounds of LYRM03 and Bestatin had inhibitory activities to aminopeptidase N of tumor cells. Fluorescein-labeled FITC-LYRM03
and FITC-Bestatin had different binding activities to tumor cells.Conclusion:There was consistency between binding activities of tumor
cells and inhibitory activities of aminopeptidase N inhibitors. |
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