| 张健 刘洪羽 杨薇薇 李春红 郑芳
蒋淑婉 李云 刘端阳 曲雪梅 陈鹤.FAP和Rho 家族蛋白在卵巢癌侵袭迁移中的作用[J].,2014,14(4):624-627 |
| FAP和Rho 家族蛋白在卵巢癌侵袭迁移中的作用 |
| FAP and Rho-GTPases on the Invasion and Metastasisof Ovarian Cancer |
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| DOI： |
| 中文关键词: 成纤维细胞激活蛋白 肿瘤转移 卵巢癌细胞系HO-8910PM RhoA/ROCK Rac1-GTP |
| 英文关键词: Fibroblast activation protein (FAP) Metastasis and invasion Ovarian cancer cell line HO-8910PM RhoA/ROCK Rac1-GTP |
| 基金项目:黑龙江省自然科学基金项目（D201049） |
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| 中文摘要: |
| 目的：明确FAP 是否通过RhoA/ROCK、Rac1-GTP 通路发挥促增殖、侵袭和迁移作用。方法：用MTT 实验，Transwell 实验
和迁移实验检测FAP、RhoA/ROCK、Rac1-GTP 对卵巢癌细胞系HO-8910PM 的增殖，侵袭和迁移的影响。结果：1、MTT 法,迁移和
侵袭实验证实用Y-27632 抑制RhoA/ROCK 途径能够促进卵巢癌细胞的增殖、迁移和侵袭，与FAP 联合作用时促进作用增强。
2、MTT 法, 迁移和侵袭实验证实NSC23766 抑制Rac1 途径能够抑制卵巢癌细胞的增殖、迁移和侵袭，与FAP 联合作用使FAP 的
促进作用减弱。结论：1、RhoA/ROCK 通路抑制HO-8910PM 细胞增殖、迁移和侵袭；Rac1-GTP 促进HO-8910PM 细胞增殖、迁移
和侵袭。2、FAP不是通过RhoA/ROCK而是通过Rac1-GTP 信号通路在HO-8910PM细胞发挥促增殖、迁移和侵袭作用的。 |
| 英文摘要: |
| Objective:To determine whether the FAP promotes the ability of proliferation, invasion and migration of HO-8901PM
cells by the RhoA/ROCK, Rac1-GTP enzymes.Methods:by detection of FAP using the transwell assay, MTT assay and migration assay,
RhoA / ROCK, Rac1-GTP on ovarian cancer cell line HO-8910PMproliferation, invasion and migration.Results:1, MTT, migration and
invasion experiments confirmed with Y-27632 inhibited RhoA / ROCK pathway can promote ovarian cancer cell proliferation, migration
and invasion, FAP facilitating role was enhanced when combined with Y-27632. 2, MTT method, migration and invasion experiments
confirmed NSC23766 inhibited Rac1 pathway to inhibit ovarian cancer cell proliferation, migration and invasion, the promoting role of
FAP was weakened when combined with NSC23766.Conclusion:1. RhoA / ROCK pathway inhibition of HO-8910PM cell
proliferation, migration and invasion; Rac1-GTP promote HO-8910PMcell proliferation, migration and invasion.2. not by RhoA/ ROCK
but Rac1-GTP signaling pathway, FAP plays a role in HO-8910PMcells proliferation, migration and invasion. |
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