| ABSTRACT Objective:To observe the effect of pulsed electromagnetic fields (PEMF) on the bone morphology and serum markers
of rats with disuse osteoporosis (DOP) and investigate the role and mechanism of PEMF in the treatment of DOP. Methods:Female SD
rats weighed 250-280 g were selected and randomly divided into 4 groups, normal control group (INT group), the disuse model group
(DOP group), drug treatment group (ALN group) and pulsed electromagnetic field group (PEMF group), 20 rats in each group, Excep the
normal control group, the rat model of disuse osteoporosis was established through an improved tibia the end of a fixed legal system
dynamic model on the rest rats. The ALN group was fed alendronate (1 mg·kg-1·d-1) treatment, the PEMF group was treated by 40 min·
d-1 PEMF the morphology of the bone tissue of rats in each group were observed 2, 4, 8 and 12 weeks after treatment. Results:After 2
weeks' treatment, compared with the INT group, the serum calcium of other group had no significant difference, the phosphorus was
significantly lower (P<0.05 or P<0.01), osteocalcin (BGP), alkaline phosphatase (ALT), tartrate-resistantphosphatase (TRAP) were
significantly higher P<0.01. After 4 weeks' treatment compared with ALN group the BGP and ALT of PEMF group were significantly
increased P<0.01; compared with DOP group, ALN group bone trabeculae was neat, trabecular spacing, reticular formation fracture.
After 8 weeks' treatment, compared with the DOP group, the ALP, TRAP of other groups reduced P<0.01, compared with the ALN
group, the BGP, ALT of PEMF group were significantly increased P<0.01. After 12 weeks' treatment, compared with the DOP group, the
BGP, ALP, TRAP of other groups reduced p<0.05 or P<0.01, compared with the drug treatment group, BGP, ALT, TRAP of the PEMF
group were significantly increased P<0.05 or P<0.01. Compared with the ALN group,the trabecular arranged neat and orderly, the
number of trabecular bone increased, the network structure was complete, the volume of trabecular increased, the thickness was
thickening. Conclusion:PEMF promoted the osteoblast function by enhancing the bone formation, and reduced osteoclast to inhibition
bone resorption,which could treat the disuse osteoporosis disease. |
