| Objective:Cancer-testis antigens（CTA）are a series of antigens which mostly expressed in testis and cancer cells, and
have strong capacity of inducing humoral and cellular immunity. They are promising as vaccine to tumor. In this investigation, we detect
the expression and regulation of CT antigens in A549 cell line. Methods:Nine kinds of CT antigens are detected of their expression in
A549 cell line using the method of RT-PCR. Also the role of TGF-beta1 and five kinds of chemotherapy medicine playing in the regulation
of CT antigens is investigated.Results:The results show that MAGEA1, MAGEA3, MAGEC1, HCA661 express in A549 cell line.
TGF茁1 down-regulates the expression of HCA661, but has no effect on MAGEA1, MAGEA3 and MAGEC1. Epirubicin down-regulates
the expression of MAGEA1, MAGEA3 and MAGEC1, but have no effect on HCA661.Conclusion:MAGEA1, MAGEA3, MAGEC1,
HCA661 stably express in A549 lung cancer cell line. For 48 hours, TGF-茁1 down-regulate the expression of HCA661, MAGEA1,
MAGEA3, MAGEC1, unchanged. Epirubicin down-regulate the expression of MAGEA1, MAGEA3 and MAGEC1, but it does not
affect HCA661. Carboplatin, paclitaxel, irinotecan, etoposide have no CT antigen expression regulation. Our study laid the basis for
further clarification of the mechanism of CTA expression regulation and provided feasibility of HCA661 as a new target for clinical
diagnosis and treatment. |
