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目的：探讨CXCR4 与MMP-9 在膀胱移行细胞癌中表达的相关性及其临床意义。方法：采用免疫组化SP 法与半定量RT-P CR 检测40 例膀胱移行细胞癌组织及10 例正常膀胱粘膜组织中CXCR4 和MMP-9 蛋白及mRNA 的表达情况，分析膀胱移行细胞癌组织中CXCR4 和MMP-9 表达的相关性，分析二者与临床病理特征的关系。结果：膀胱移行细胞癌组织中CXCR4 蛋白表达率为77.5%，mRNA 相对含量为0.777±0.044；其中浸润深度达肌层者表达率为100%，mRNA 相对含量为0.790±0.049；局限在粘膜下层者表达率为50%，mRNA 相对含量为0.660±0.052；二者之间差异有统计学意义。MMP-9 在膀胱移行细胞癌组织中的表达率为80.0%，mRNA 相对含量为0.850±0.079，其中浸润深度达肌层者表达率为95.5%，mRNA 相对含量为0.854±0.070，局限在粘膜下层者表达率为61.1%，mRNA 相对含量为0.758±0.092，二者之间差异有统计学意义。膀胱移行细胞癌组织中CXCR4 及 MMP-9 蛋白阳性表达呈正相关关系(γ＝0.479，P＜0.05)。MMP-9 与肿瘤组织学分级有关，与患者的性别、年龄无关；而CXCR4 的表达与肿瘤组织学分级及患者的性别、年龄均无关。结论：CXCR4 和MMP-9 表达与膀胱移行细胞癌的发生和浸润密切相关，通过干预CXCR4 和MMP-9 的活性可能成为治疗膀胱移行细胞癌的新靶点。

英文摘要:

Objective: To investigate the expression and clinical significance of chemokine receptor CXCR4 and MMP-9 (matrix metalloproteinase-9) in human bladder transitional cell carcinoma (BTCC). Methods: CXCR4 and MMP-9 protein and mRNA were detected in 40 formalin-fixed paraffin-embedded and fresh BTCC tissues and 10 normal bladder mucosal tissues by immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) respectively. The association was analyzed between the expressions of CXCR4 and MMP-9, as well as their relationships to the clinicopathologic significance of BTCC. Results: The positive rate of expression of CXCR4 and MMP-9 protein and relative level of mRNA in BTCC tissues were significantly higher than those of the normal bladder mucosal tissues (CXCR4: 77.5% vs. 20%, 0.777±0.044 vs. 0.623±0.096; MMP-9: 80.0% vs. 20%, 0.850±0.079 vs. 0.751±0.049). Further more, the positive rate of expression of CXCR4 and MMP-9 protein and relative level of mRNA in the muscle-invasive tumors were significantly higher than those of confined within submucosa too (CXCR4: 100% vs. 50%, 0.790±0.049 vs. 0.660± 0.052; MMP-9: 95.5% vs. 61.1%, 0.854±0.070 vs. 0.758±0.092). The expression of CXCR4 protein was significantly positively correlated with that of MMP-9 protein (P<0.05). Nevertheless, the expression of CXCR4 among different histological grades did not have statistical significance while the expression of MMP-9 did. Both of the expression of CXCR4 and MMP-9 had no correlation with the sex and age of the patients. Conclusion: Our results suggest that CXCR4 and MMP-9 expression is highly correlated with the occurring and invasion of BTCC, and indicate that CXCR4 and MMP-9 could be a new efficient target to treat bladder cancer.

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