| 蒋易1 沈桢巍1 雷撼2 马少林1 白建文3△.VEGF 对大鼠脑缺血再灌注损伤的预防作用及其机制研究[J].,2012,12(27):5251-5254 |
| VEGF 对大鼠脑缺血再灌注损伤的预防作用及其机制研究 |
| The Neuroprotective Effect of VEGF on Ischemia-Reperfusion CerebralInjury in Rats* |
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| DOI： |
| 中文关键词: 血管内皮生长因子 热休克蛋白70 脑缺血再灌注损伤 |
| 英文关键词: VEGF TUNEL HSP-70 Cerebral ischemia-reperfusion injury |
| 基金项目:浦东新区社发局课题(PW2005A-10) |
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| 中文摘要: |
| 目的：研究局灶性脑缺血再灌注后细胞凋亡、HSP70 蛋白表达时空规律以及外源VEGF 及VEGF 抗体对它们的影响，探讨
VEGF 对缺血再灌注损伤的保护作用及其机制。方法：采用原位末端标记(TUNEL)、免疫组化方法，研究局灶性脑缺血再灌注后细
胞凋亡数及HSP70 蛋白表达时空分布，采用脑表面使用VEGF 及侧脑室注射VEGF 抗体，观察内外源VEGF 对它们的影响。结
果：VEGF 抗体能显著增加缺血侧脑组织凋亡细胞数(再灌注12h-7d)及HSP70 表达量(再灌注1-3d)，而外源VEGF 因子能显著减
少同侧脑组织凋亡细胞(再灌注全程)及HSP70 表达量(再灌注1-3d)。结论：VEGF 因子可抑制缺血脑组织细胞凋亡及HSP70 表达
量，提示VEGF 参与保护缺血性脑损伤。 |
| 英文摘要: |
| Objective: To explore the implications of HSP70 and apoptosis in rat brain following ischemia-reperfusion and to
study the neuroprotective effect of VEGF on ischemia-reperfusion cerebral injury in rats. Methods: HSP70 protein and apoptosis cells
were detected by immunohistochemistry and TUNEL methods in ischemia-reperfusion rat brain. Simultaneously, the effect of exogeous
VEGF protein and VEGF antibody was observed. Results: TUNEL and immunohistochemical analysis for HSP70 showed an
amelioration of stalnings at 24 and 72 hours after reperfusion with VEGF treatment, which indicated reduction of neuronal damage. On
the contrary, application of VEGF antibody produced reverse results as compared with cases with VEGF treatment after reperfusion.
Conclusion: Treatment with topical VEGF application significantly reduced ischemic brain damage, while antagonism of VEGF
enhanced ischemic-reperfusion-related brain injury. |
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