| 田国梅赵长久△ 付鹏栾厦张月红吴琼.MDM2 反义寡核苷酸联合紫杉醇对乳腺癌MCF-7 细胞株的作用[J].,2012,12(25):4801-4804 |
| MDM2 反义寡核苷酸联合紫杉醇对乳腺癌MCF-7 细胞株的作用 |
| Effects of MDM2 Antisense Oligonucleotide Combined with Paclitaxelon Human Breast Cancer Cells MCF-7 |
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| DOI： |
| 中文关键词: MDM2 基因 反义寡核苷酸 紫杉醇 乳腺癌MCF-7 细胞 |
| 英文关键词: MDM2 Gene Antisense Oligonucleotidel Paclitaxel Breast Cancer Cell Line MCF-7 |
| 基金项目:国家自然科学基金（81171362） |
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| 中文摘要: |
| 目的：探讨靶向MDM2 反义寡核苷酸（ASON）联合紫杉醇对乳腺癌MCF-7 细胞株的影响。方法：合成一段与MDM2 mRNA
特异性结合的反义寡核苷酸和与反义寡核苷酸有4 个碱基不同的的错义寡核苷酸（MON），脂质体2000 介导不同浓度的MDM2
ASON 转染MCF-7 乳腺癌细胞系，转染的乳腺癌细胞通过1 μmol/L 紫杉醇药物处理后，采用RT-PCR 和Western Blot 方法检测
MDM2 ASON 联合紫杉醇的协同作用及对乳腺癌MCF-7 细胞株的抑制效率，MTT 观察给药后MCF-7 细胞的增殖能力和药物敏
感性。结果：MDM2 反义寡核苷酸联合紫杉醇明显下调MDM2 mRNA 及MDM2 蛋白表达水平，抑制MCF-7 细胞的生长，随着
MDM2 ASON 浓度的增加，MDM2 表达越来越低，协同作用越来越强，呈剂量依赖关系，A500 联合紫杉醇的协同作用最明显，
MTT 显示紫杉醇处理的转染MCF-7 细胞增殖抑制率明显增高，A500 抑制增殖作用最明显，抑制率达（13.0±0.84）%。结论：不同
浓度MDM2 ASON 转染后的乳腺癌MCF-7 细胞，等浓度紫杉醇处理后，乳腺癌MCF-7 细胞MDM2 表达明显降低，细胞凋亡增
加，，MDM2 ASON 联合紫杉醇对MCF-7 细胞有协同作用，提高了乳腺癌MCF-7 细胞对紫杉醇的药物敏感性。 |
| 英文摘要: |
| Objective: To investigate the effects of the MDM2 antisense oligonucleotide (ASON) combined with Paclitaxel on human
breast cancer cells MCF-7. Methods: The synthesis of antisense oligonucleotides specific binding of MDM2 mRNA and missense
oligonucleotides(MON) different from four bases, different concentrations of MDM2 ASON mediated by Lipofectamine 2000 transfected
MCF-7 breast cancer cell lines, breast cancer cells transfected by 1 μmol/L paclitaxel treatment.The expression of MDM2 mRNA and
protein was determined by RT-PCR and Western blotting, To detect synergies of MDM2 ASON combined with paclitaxel and the inhibition
efficiency of breast cancer cells MCF-7, the proliferation of MCF-7 cell to paclitaxe and chemosensitivity were observed by MTT assay.
Results: The antisense oligonucleotide combined with Paclitaxel efficiently down-regulated MDM2 mRNA and protein expression,
inhibit the growth ofMCF-7 cells. MDM2 expression was getting lower and lower with the increase of the concentration ofMDM2 ASON
growing in a dose dependent relationship, the synergy of the A500 combined with paclitaxel was the most obvious. MTT showed that
proliferation inhibition rate of MCF-7 cell transfected to paclitaxel increased significantly, A500 was the most significant effect, inhibition
rate was (13.0 ± 0.84)%. Conclusion: Human breast cancer cell MCF-7 transfected was treated by a concentration of paclitaxel,
MDM2 expression was significantly decreased, increased apoptosis, MDM2 ASON combined with paclitaxel on MCF-7 cells had a synergistic
effect, improved the sensitivity of breast cancer MCF-7 cells to paclitaxel. |
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