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目的：研究缺氧诱导因子-1α(HIF-1α)在人垂体腺瘤中的表达，对HIF-1α 蛋白表达与肿瘤分级进行相关性分析，探讨其表达水平与垂体腺瘤侵袭性的关系。方法：集影像学检查、内分泌学检查及病理诊断的垂体腺瘤60 例，分为侵袭组和非侵袭组，其中侵袭组36 例，非侵袭组24 例；对照组正常脑组织5 例。免疫组织化学技术检测HIF-1α 蛋白的表达，结合临床资料进行统计学分析。分析垂体腺瘤HIF-1α 蛋白的表达水平并与对照组进行比较，比较侵袭组和非侵袭组垂体腺瘤之间HIF-1α 蛋白表达水平的差异。结果：HIF-1α 蛋白在垂体腺瘤中的表达明显高于对照组，二者比较，x2 0.05,1 =12.392，P＜0.001，有显著性差异；侵袭组HIF-1α 蛋白的表达较非侵袭组显著增高，二者比较，x2 0.05,1 =24.658，P < 0.001，有显著性差异。结论：HIF-1α 是垂体腺瘤侵袭过程中的重要调控因子，与垂体腺瘤的大小、分级以及侵袭性密切相关，其作用机制有待进一步研究，其表达程度可用作垂体腺瘤预后的评估指标，为垂体腺瘤术后的复发以及相应的辅助治疗提供判断依据。有可能为人们靶向肿瘤缺氧来开发新药物提供新的作用靶点。

英文摘要:

Objective: To study the protein expressions of hypoxia inducible factor 1α(HIF-1α) in pituitary adenomas, and the correlation of protein expression HIF-1α with the grade and volume of pituitary adenomas, and probe the relationship between proteins and invasion of pituitary adenomas. Methods: Sixty surgical samples of pituitary adenomas were diagnosed by examine of biology and endocrinology, divided into invasive and non-invasive pituitary adenomas groups and in 5 normal sham patients. Analysis of pituitary adenoma HIF-1 α protein expression level and compared with the control group, more invasive group and the attack group between pituitary adenoma HIF-1 α protein expression level of the differences. Results: The Protein expression of HIF-1α in Pituitary adenomas group were significantly higher than sham group (x2 0.05,1 =12.392，P＜0.001); HIF-1α in invasive Pituitary adenomas group were significantly higher than non-invasive Pituitary adenomas group (x2 0.05,1=24.658, P<0.001). Conclusion: Hypoxia- inducible - 1 alpha may be an important regulatory factors in pituitary adenoma' invasive, and the size and grading with the invasive closely related, the mechanism of its action research further.

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