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目的：观察COPD 大鼠模型中瘦素（leptin）、白细胞介素8（IL-8）的表达情况，分析其相关性，探讨leptin 在COPD 发生发展中的作用及意义。方法：36 只雄性SD 大鼠随机分为①健康对照组；②COPD 模型1 组：分别于第（1、14）d 经气管内注入内毒素 200ug,熏5%香烟（第1、14d 除外），2h/d,共4 周；③COPD 模型2 组：单纯熏5%香烟2h/d，共12 周。观察肺组织病理变化，免疫组化法测定leptin、IL-8 在支气管肺组织的表达情况，放免法测定血清leptin 及IL-8 浓度。结果：细胞因子leptin 及炎性因子IL-8 在支气管肺组织阳性表达。LPS 联合熏烟诱导COPD1 组支气管肺组织中leptin（52.67±04.72）和IL-8（59.56±3.94）表达较单纯熏烟诱导COPD2 组leptin（38.89±2.57）和IL-8（55.22±3.42）表达明显升高，P<0.05，两组COPD 大鼠模型支气管肺组织中leptin 及 IL-8 表达较正常对照组leptin（16.90±1.52）和IL-8（28.00±4.24）表达均明显增高，P<0.05，COPD1 组血清中leptin（3.26±0.95） ng/mL和IL-8（107.51±13.38）pg/mL较COPD2 组中leptin（2.42±0.69）ng/mL和IL-8((94.07±11.20）pg/mL明显增高，P<0.05，两组 COPD 大鼠模型血清中leptin 及IL-8 浓度较正常对照组leptin（0.95±0.56）ng/mL和IL-8（39.48±6.35）pg/mL浓度显著升高，P<0. 05。血清中Leptin 与IL-8 表达水平呈显著正相关（r 值分别为0.72 0.67 0.84 均P<0.05）。经过q 检验，两两之间比较均有统计学意义。结论：leptin 和IL-8 均参与COPD 炎症反应过程，并且具有相关性，LPS 促进二者的表达。

英文摘要:

Objective: To investigate the expression matter of leptin and IL-8 in rat models of COPD, and analyse their relevance, investigate the value of leptin in the development of COPD. Methods: 36 Male SD rats were randomly divided into 3 groups: (1) Control group: untreated rats;（2）COPD1: rats were intratracheally administered LPS (200 μg) on d 1 and 14 and exposed to 5% smoke for 2 h daily for 4 consecutive weeks;（3）COPD2: rats were exposed to 5% smoke for 2 h daily for 12 consecutive weeks. To observe the pathological changes of the lung tissues, and take immunohistochemistry to determine leptin and IL-8 levels expressed in bronchial lung tissue. Serum leptin and IL-8 concentration was examined by radio-immunity method. Results: The cell factor leptin and inflammatory factor IL-8 in bronchial lung tissue were positive expressed. The expression of leptin ( 52.67±04.72)and IL-8 (59.56±3.94) in COPD 1 group was higher than that in COPD2 group leptin(38.89±2.57）and IL-8(55.22±3.42), P<0.05; Both leptin and IL-8 expression in two COPD model groups were higher than that in the normal group (16.90±1.52) and (28.00±4.24), P<0.05; In the COPD1 group, serum leptin level (3.26±0.95) ng/mL and IL-8(107.51±13.38) levels were markedly higher than that in the COPD2 group(2.42±0.69)ng/ml and (94.07±11.20) pg/mL, P < 0.05, and these levels in both COPD groups increased dramatically compared with that in controls (0.95±0.56)ng/mL and IL-8 (39.48±6.35) pg/mL, P<0.05. There was negative correlation between leptin and IL-8 expression（r=0.72 0.67 0.84 all P<0.05）in blood. The comparison between the two groups had statistical significance after inspection. Conclusion: Leptin and IL-8 play important roles in the occurrence and development of COPD, which is correlated with each other. In addition, LPS facilitates the expression of leptin and IL-8.

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