| 燕琳桂燕琳桂伶俐△ 张传汉张玥邹姮婧李大佳石小云伶俐△ 张传汉张玥邹姮婧李大佳石小云.建立新生大鼠吸入麻醉模型及异氟醚对其海马凋亡的影响[J].,2012,12(24):4601-4605 |
| 建立新生大鼠吸入麻醉模型及异氟醚对其海马凋亡的影响 |
| Establish a Model for Evaluating the Effects of Isoflurane on the Apotosis inHippocampal Cells of Neonatal Rats |
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| DOI： |
| 中文关键词: 异氟醚 大鼠 吸入麻醉 |
| 英文关键词: Isoflurane Neonatal rats Anesthesia Inhalation |
| 基金项目:高等学校博士学科点专项科研基金（200804871046）；华中科技大学同济医学院院基金 |
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| 中文摘要: |
| 目的：建立新生大鼠吸入麻醉模型并探讨吸入麻醉药异氟醚对其海马凋亡的影响。方法：Penlon Prima SP 麻醉机、异氟醚挥
发罐及自制带进出气口的麻醉小室。共55 只7 日龄的SD 大鼠用于实验。将其中35 只大鼠随机分为7 组（n=5）。实验组（Ⅰ-Ⅵ
组）异氟醚挥发罐刻度分别为0.125%，0.25%，0.5%，1%，1.5%，2%；新生大鼠置于自制密封麻醉小室内，分别通入含上述异氟醚浓
度的混合气体。对照组（第Ⅶ组）给予未混合异氟醚的30%的氧气。将小室安放于37℃恒温箱内。调节气体流量2L/min。实验组
于通入气体5, 10, 15, 30, 90, 180, 360 min（T1-7）时于小室出口处抽取10mL 气体，采用气相色谱法测定麻醉小室内异氟醚浓度。
于通入气体360 min（T7）自新生大鼠左心室采血行血气分析；另取SD 大鼠20 只，随机分为对照组(C 组,n=10)，1.5%异氟醚组(I
组,n=10)，按上述方法建立异氟醚吸入麻醉模型，麻醉结束后2h 处死大鼠，采用免疫组织化学法观察C 组和I 组大鼠大脑海马区
Active caspase-3 的表达。结果：①麻醉小室出口异氟醚浓度（Y） 与麻醉机挥发罐异氟醚浓度（X） 的直线回归方程为Y=1.
5472X-0.0575（r=0.9993）。②血气分析结果显示：Ⅰ-Ⅵ组与Ⅶ组血气分析组间差异无统计学意义（P＞0.05）。③免疫组化结果显
示：与C 组相比，I 组大鼠海马Active caspase-3 明显增加，差异有统计学意义（P＜0.05）。结论：通过麻醉机、异氟醚挥发罐及自制
密封带进出气口的麻醉小室成功建立了新生大鼠异氟醚麻醉模型；为进一步研究异氟醚及相关吸入麻醉药对突触发生期的神经
毒性提供了实验基础。 |
| 英文摘要: |
| Objective: To establish a model of applying inhalation anesthesia for neonatal rats and evaluate the reliability of thisrats is successfully established using an anaesthesia apparatus, a calibrated vaporizer of isoflurane, a domestic air-tight chamber with an
air-scoop and an air-out，which may provided experimental basis for further studies of inhalation anesthetics such as isoflurane on the
triggering of synaptic neurotoxicity.
model, then to investigate the apotosis of isoflurane on the hippocampal cells of neonatal rats. Methods: Connected the anaesthesia
mechine equipped with a calibrated vaporizer of isoflurane, to the domestic air-tight chamber with an air-scoop and an air-out to form
anesthesia pipeline. 55 seven-day-old male and female Sprague Dawley rats were used for the experiment. 35 SD rats were divided into
seven randomly selected groups （n=5）. According to the different concentrations of the isoflurane in the air stream, the mixture gas were
divided into six groups as follows: (1)Experiment groups: 0.125% (groupⅠ), 0.25% (groupⅡ), 0.5%(group Ⅲ), 1% (group Ⅳ), 1.5%
(group Ⅴ ), 2% (group Ⅵ ); (2) Control groups (group Ⅶ ): air stream composed only of 70% nitrogen mixed 30% oxygen. The
experimental rats were exposed to isoflurane in the air-tight chamber, which was placed inside a constant temperature incubator, setting
up at 37℃. Isoflurane was continuously delivered into chamber through inlet with an air stream (70% nitrogen +30% oxygen) containing
desired anesthetic concentration using a calibrated vaporizer (before each test, the mixture gas was flow through the chamber several
minutes). The mixture gas samples in chamber of each experiment group were taken at 5min, 10min, 15min, 30min, 90min, 180min and
360min after isoflurane intervention. Isoflurane concentrations in the chamber were measure by gas chromatograph. The samples of both
the experiment groups and the control group were taken at the end of anesthesia for blood gas analysis. The other 20 seven-day-old SD
rats were divided into two randomly selected groups(n=10), control group and 1.5%isoflurane group, to establish the isoflurane inhalation
model as above-mentioned, and using immunohistochemistry to detect the expression of Active caspase-3. Results: ①Whereafter the
isoflurane concentrations in them became linearly dependented, the linear dependence equation of them is Y=1.5472X-0.0575(r=0.9993).
②The values of pH, PO2, PCO2, BE, HCO3
- and SaO2 between experiment groups and control groups had no significant difference at the
end of anesthesia time point (P＞0.05). ③Compared with C group, the Active caspase-3 immono-stain positive cells were significantly
increased in the I group in the hippocampal areas (P＜0.05). Conclusion: The model for evaluating the effects of isoflurane on neonatal |
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