| 车红花1 张明雪2△ 何伟3 侯平2 顾平4.益气活血中药复方对CVB3 大鼠心肌细胞感染模型ATP6 基因
表达的影响[J].,2012,12(23):4412-4415 |
| 益气活血中药复方对CVB3 大鼠心肌细胞感染模型ATP6 基因
表达的影响 |
| The Influence of Supplementing Qi and Activating Blood Circulation ChineseHerbal Compound to the Genetic Expression of CVB3 Rat Cardiac MuscleCell Infection Model ATP Synthase F0 Subunit 6 |
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| DOI： |
| 中文关键词: 病毒性心肌炎 益气活血中药复方 抑制性消减杂交技术 ATP6 |
| 英文关键词: Viral myocarditis (VMC) Supplementing Qi and activating blood circulation Chinese Herbal Compound Suppression
subtractive hybridization (SSH) ATP synthase F0 subunit 6 |
| 基金项目:国家自然科学基金面上项目（30873399）；教育部博士点基金(20070162002)；
辽宁省高等学校优秀人才支持计划 |
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| 中文摘要: |
| 目的：研究益气活血中药复方对CVB3 大鼠心肌细胞感染模型ATP6（ATP synthase F0 subunit 6）基因表达的作用机制。方法：
本实验用新生2-3dWistar 大鼠心肌细胞,建立CVB3 病毒感染模型，通过改良的抑制性消减杂交技术（SSH），克隆了受CVB3 攻击
的心肌细胞中被中药（益气活血中药复方）调控的基因。结果：ATP 6 基因，在中药组中高表达，而病毒组中表达减弱或抑制。结论：
益气活血中药复方能影响受CVB3 病毒攻击的宿主细胞ATP6 基因的表达，有效保护心肌、阻断病程进度，从而实现治疗病毒性
心肌炎的目的。 |
| 英文摘要: |
| Objective: This study is about the mechanism of supplementing Qi and activating blood circulation Chinese Herbal
Compound to the genetic expression of CVB3 rat cardiac muscle cell infection model ATP synthase F0 subunit 6. Methods: This
experiment set CVB3 infection model by the new 2-3d rats' cardiac muscle cells, used the improved suppression subtractive hybridization
(SSH), and cloned genes of host cells attacked by CVB3 and regulated by supplementing Qi and activating blood circulation Chinese
Herbal Compound. Results: The expression of ATP synthase F0 subunit 6 in Chinese medicine group present high, but in virus group
suppressed. Conclusion: Supplementing Qi and activating blood circulation Chinese Herbal Compound can effect on the genetic
expression of ATP synthase F0 subunit 6 attacked by CVB3, protect myocardial effectively and block the course of disease, so that
achieve the purpose of treating viral myocarditis. |
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