| 魏蓓蓓刘一浓秦明杰许观萍吴飞.静电纺丝纳米纤维体外释放与蛋白活性的研究[J].,2012,12(20):3809-3811 |
| 静电纺丝纳米纤维体外释放与蛋白活性的研究 |
| In Vitro Release and Protein Bioactivity Study of Electrospun Nanofibers |
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| DOI： |
| 中文关键词: 纳米纤维 体外释放 蛋白活性 |
| 英文关键词: Nanofibers In Vitro Release Protein Bioactivity |
| 基金项目:国家自然科学基金项目（81102406） |
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| 摘要点击次数: 715 |
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| 中文摘要: |
| 目的：通过选择不同的模型蛋白，探讨准确的研究静电纺丝纳米纤维支架的体外释放和快速的测定蛋白活性的方法。方
法：通过O/W 乳液法静电纺丝制备纳米纤维，并用扫描电镜对纳米纤维表面进行了表征。以GM-CSF 为模型蛋白，采用ELISA
双抗体夹心法考察纤维的体外释放行为；以BSA 为模型蛋白，用SEC-HPLC 比较纤维制备前后蛋白的聚集情况；以β- 半乳糖苷
酶为模型蛋白，用ONPG 法比较纤维制备前后酶的催化活性。结果：纤维表面平滑，直径均一，呈现互相连通的三维网状结构。纤
维在5 天内释放90%以上；纤维中回收的BSA 单体比例为66.53%；β- 半乳糖苷酶在纤维中的催化活性保持原活性的3.37%。结
论：通过选择不同的模型蛋白，能够准确的测定静电纺丝纤维的体外释放，快速的考察纤维中的蛋白活性，对于更好的研究蛋白
药物纳米纤维支架具有重要的参考价值。 |
| 英文摘要: |
| Objective: To investigate the method of detecting the in vitro release and protein bioactivity of electrospun nanofibers
with different model proteins. Methods: Nanofibers were prepared byW/O emulsion electrospinning. ELISA method was used to investigate
the in vitro release of nanofibers with GM-CSF as model protein. SEC-HPLC was used to test the protein aggregates with BSA as
model protein. ONPG method was used to test protein bioactivity with β-galactosidase as model protein. Results: The nanofibers were
smooth and uniform. GM-CSF can be released more than 90% in 5 days; BSA monomer preserved 66.53% after recovering from
nanofibers; β-galactosidase bioactivity preserved 3.37% after recovering from nanofibers. Conclusion: The in vitro release and protein
bioactivity of electrospun nanofibers can be tested fast and accurate with different model proteins. |
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