| 秦向英1 乔万臣2 慕璐岩2 宋玉文2 汤加斌2 董白晶2
胡炜2 王策2 赵天书2 李利2 赵佳鑫2 刘晓谦1.Stathmin 在微血管内皮细胞的表达与胶质瘤恶性程度关系[J].,2012,12(19):3628-3631 |
| Stathmin 在微血管内皮细胞的表达与胶质瘤恶性程度关系 |
| Stathmin Expression in Microvascular Endothelial Cells Is Associated withGlioma Grade |
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| DOI： |
| 中文关键词: 脑胶质瘤 Stathmin 微血管内皮细胞 表达 |
| 英文关键词: Glioma Stathmin Endothelial cells Expression |
| 基金项目:黑龙江省人力资源和社会保障厅归国留学人员重点资助项目 |
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| 中文摘要: |
| 目的：检测Stathmin 在正常脑组织及不同级别胶质瘤微血管内皮细胞中的表达情况。方法：利用结合CD105 单克隆抗体的
免疫磁珠内皮细胞分选系统特异性分选出68 例胶质瘤微血管内皮细胞（其中低级别胶质瘤（WHO 分级Ⅰ-Ⅱ）24 例，高级别胶质
瘤（WHO 分级Ⅲ-Ⅳ）44 例）和20 例正常脑组织微血管内皮细胞。应用免疫组化、RT-PCR 和Western blot 检测Stathmin 在胶质瘤
微血管内皮细胞和正常脑组织微血管内皮细胞中的表达。结果：免疫组化证实Stathmin 在正常脑组织微血管内皮细胞、低级别胶
质瘤微血管内皮细胞和高级别胶质瘤微血管内皮细胞的表达百分率分别是20%，66%和95%( P<0.05)。RT-PCR 和Western blot
法检测显示，Stathmin 在胶质瘤微血管内皮细胞中的表达明显增高。低级别胶质瘤组、高级别胶质瘤组分别与正常组比较，均有显
著性差异(P<0.01)；且低级别胶质瘤组与高级别胶质瘤组比较，有显著性差异(P<0.01)，随着胶质瘤恶性程度的增加，Stathmin 表达
上调，具有统计学意义。结论：Stathmin 在脑胶质瘤微血管内皮细胞中表达随肿瘤恶性程度增高而增加，可能为脑胶质瘤的生物
治疗提供一个新靶点。 |
| 英文摘要: |
| Objective: To investigate stathminin expression in human gliomas and normal brain specimans derived microvessels
endothelial cells (GDMEC). Methods: Microvascular endothelial cells from human gliomas (GBMEC, n=68) and normal brain (NBMEC,
n=20) were purified by incubating with magnetic beads coated with anti-CD105 antibody. There were 24 cases of low grade gliomas
(WHOⅠ-Ⅱgrade) and 44 high grade gliomas (WHO Ⅲ-Ⅳgrade). Expression rates and cellular distribution of stathmin in GDMEC and
NBMEC were also investigated by immunohistochemistry. Semiquantitative RT-PCR and Western blot were used for comparing the expression
of stathmin in GDMEC and NBMEC. Results: Immunohistochemical analysis demonstrated that the rates of stathmin expression
in NBMEC, low grade GDMEC, and high grade GDMEC were 20%, 66%, and 95%, respectively (P<0.05). The expression of stathmin
in GDMEC was higher than that in NBMEC. The difference between Ⅰ-Ⅱgrade, Ⅲ-Ⅳgrade and normal human brain tissues were
significant (P<0.01) respectively. The difference betweenⅠ-Ⅱgrade and Ⅲ-Ⅳ grade was significant(P<0.01). Conclusions: With the rise
of pathological grades of glioma, the expression of stathmin increased, and it may represent a novel tumor-related target for the therapy of
malignant gliomas. |
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