| 闵宁斌1 唐治国2 黎璞3 王丽萍1 党亚茹1.银杏叶提取物对新生SD 乳鼠心肌细胞缺氧损伤的影响[J].,2012,12(18):3464-3468 |
| 银杏叶提取物对新生SD 乳鼠心肌细胞缺氧损伤的影响 |
| The Effect of Extract of Ginkgo Biloba Leaf on Neonatal SD Rat MyocardialCells during Hypoxic Injury |
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| DOI： |
| 中文关键词: 银杏叶提取物 SD 乳鼠 缺氧 凋亡 |
| 英文关键词: Extract of ginkgo biloba leaf SD rat Hypoxia Apoptosis |
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| 中文摘要: |
| 目的：研究银杏叶提取物对缺氧状态下新生SD 乳鼠心肌细胞的影响及其可能机制。方法：新生1 天SD 乳鼠心肌细胞原代
培养并利用氮气培养箱模拟低氧构建乳鼠心肌缺氧体外模型。分为3 组处理：对照组，缺氧组，缺氧+ 药物拮抗组。缺氧时间为
12 h，通过免疫组化等检测方法，观察各组心肌细胞的损伤情况及心肌Bcl-2、Bax 蛋白表达情况。结果：缺氧可以造成新生SD 乳
鼠心肌细胞凋亡的发生（hypoxia: 75.21 %±1.21 %, control: 1.38 %±0.45 %, P<0.05, n=20），并导致其表达凋亡抑制因子Bcl-2 蛋
白水平的显著降低（0.125 fold VS control group, P<0.05），促细胞凋亡因子Bax 蛋白水平显著升高（3.011fold VS control group,
P<0.05）；而银杏叶提取物作用后可明显逆转新生SD 乳鼠心肌细胞凋亡的发生（EGb761: 23.17 %±0.43 %, hypoxia: 73.13 %±
1.22 %, P<0.05, n=20）,并明显逆转Bcl-2 (5.716 fold VS hypoxia group, P<0.05)、Bax (0.273fold VS hypoxia group, P<0.05)等蛋白的
表达水平。结论：凋亡相关因子Bcl-2 和Bax 等参与缺氧致心肌损伤过程，导致心肌细胞凋亡，银杏叶提取物能降低心肌Bax 表
达，提高Bcl-2 表达，从而保护心肌细胞，抑制凋亡。 |
| 英文摘要: |
| Objective: To investigate the effect of extract of ginkgo biloba leaf on neonatal SD rat myocardial cells during hypoxic
injury and its possible mechanism. Methods: Neonatal 1 d SD rats' myocardial cells were cultured and the nitrogen incubator was used to
mimic hypoxia model in vitro. Primary cultures were divided into three groups: blank group, hypoxia group, hypoxia and medicine interference
group. For the hypoxia group and hypoxia plus medicine interference group, cells were cultured in hypoxia incubator for 12 h,
then the expression of Bcl-2 and Bax were detected by western blot and the cell apoptosis was detected with in situ TUNEL assay. Results:
The apoptosis of neonatal SD rat myocardial cells were detected after 12 h hypoxia (hypoxia: 75.21 %±1.21 %, control: 1.38 %±0.45
%, P<0.05, n=20). Meanwhile, hypoxia also reduced expression of Bcl-2 (0.125 fold VS control group, P<0.05) and elevated expression
of Bax (3.011fold VS control group, P<0.05). Moreover, the extract of ginkgo biloba leaf could significantly inhibit the apoptosis of
neonatal SD rat myocardial cells exposed to hypoxia (EGb761: 23.17 % ± 0.43 %, hypoxia: 73.13 % ± 1.22 %, P<0.05, n=20) and significantly
invert the expression of Bcl-2 (5.716 fold VS hypoxia group, P<0.05) and Bax (0.273fold VS hypoxia group, P<0.05). Conclusion:
Apoptosis-related factors such as Bcl-2 and Bax may play an important role in the process of hypoxia-induced myocardial cells injury,
which may lead to myocardial cells apoptosis. The extract of ginkgo biloba leaf could reduce the expression of Bax and elevate the expression
of Bcl-2, and accordingly protect myocardial cells from hypoxia induced apoptosis. |
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