文章摘要
徐沙沙1,2 项锋钢1,2△ 党受琴3 冉雯雯2 李宏2.NF-kB 与Slug 在非小细胞肺癌及其上皮间质转化中的作用[J].,2012,12(17):
NF-kB 与Slug 在非小细胞肺癌及其上皮间质转化中的作用
The Expression of NF-kB and Slug in NSCLC and the Effect on EMT
  
DOI:
中文关键词: NF-kB  Slug  EMT  RT-PCR  免疫组织化学  NSCLC
英文关键词: NF-κB  Slug  EMT  RT-PCR  Immunohistochemistry
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作者单位
徐沙沙1,2 项锋钢1,2△ 党受琴3 冉雯雯2 李宏2 青岛大学医学院病理学教研室 
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中文摘要:
      目的:研究核因子NF-kB 与slug 在非小细胞肺癌(NSCLC)中的表达情况、及二者与非小细胞肺癌上皮间质转化(EMT)的 关系,为非小细胞肺癌的诊断治疗提供理论依据。方法:(1)采用免疫组化PV9000 二步法测定50 例NSCLC 组织及20 例相应正 常肺组织中NF-kBP65、slug、E-cadherin 及Vimentin 蛋白表达情况。(2)采用RT-PCR 测定其中25 例NSCLC 组织及10 例相应正 常肺组织中NF-kBP65、slug 的mRNA 表达情况。结果:NSCLC 中NF-kBP65 蛋白表达量高于癌旁正常肺组织(Z=-2.370, P< 0.05),NF-kBP65mRNA 表达量明显高于癌旁正常肺组织(t=4.967,P<0.01);Slug 蛋白表达量明显高于癌旁正常肺组织(Z=-4.443, P<0.01),SlugmRNA 表达量明显高于癌旁正常肺组织(t=6.483,P<0.01)。在NF-kBP65 阳性癌组织中,E-cadherin 蛋白表达下降 (x2=5.024, P<0.05),Vimentin 蛋白表达上升(x2=4.723,P<0.05);Slug 阳性癌组织中,E-cadherin 蛋白表达下调(x2=5.984,P< 0.05),Vimentin 表达上调(x2=5.028,P<0.05)。另外,NF-kBP65 与Slug 在蛋白水平呈极显著正相关(r=0.443, P<0.01),在mRNA 水平呈显著正相关(r=0.439,P<0.05)。NF-kB 与分化程度(x2=5.024,P<0.05)、有无淋巴结转移(x2=7.933, P<0.01)及肿瘤的分期 (x2=5.614,P<0.05)有关,与性别、年龄、组织类型无明显相关性(P>0.05);Slug 与淋巴结转移(x2=6.174, P<0.05)及肿瘤的分期 (x2=7.317,P<0.01)有关,与性别、年龄、组织类型、分化程度无明显相关性(P>0.05)。结论:NF-kB、Slug 在NSCLC 中表达增强,可 能与NSCLC 的发生、发展、转移有关;并且NF-kB 与Slug 可能协同抑制E-cadherin 表达,促进Vimentin 表达,诱使NSCLC 的 EMT 发生,从而为进一步研究NSCLC 的EMT 提供理论依据。
英文摘要:
      Objective: To investigate the expression of NF-kB and Slug in NSCLC and their relationship with EMT, to provide theoretical basis to the treatment of NSCLC. Methods: (1) PV9000 two-step immunohistochemical method was used to detect the expression of NF-κBP65, Slug, E-cadherin and Vimentin in 50 NSCLC tissues and 20 adjacent normal lung tissues. (2) RT-PCR was performed to determine the expression of NF-κBP65mRNA and SlugmRNA in 25 NSCLC tissues and 10 adjacent normal lung tissues. Results: In NSCLC tissues, NF-κBP65 expression was higher than that in adjacent normal lung tissues (Z=-2.370, P<0.05); mRNA expression of NF-kBP65 was significantly higher than that in adjacent normal lung tissues (t=4.967,P<0.01); Slug expression was significantly higher than that in adjacent normal lung tissues (Z=-4.443,P<0.01); The expression of Slug was significantly higher than that in adjacent normal lung tissues (t=6.483, P<0.01). In NF-kBP65 positive NSCLC tissues, E-cadherin expression decreased (X2=5.024,P<0.05) while vimentin expression increased (X2=4.723,P<0.05). In Slug positive NSCLC tissues, E-cadherin expression decreased (X2=5.984, P<0.05) and Vimentin expression increased (X2=5.028,P<0.05). In addition, NF-kBP65 had very significantly positively correlated with Slug (r=0.443,P<0.01). NF-kB was related to differentiation degree (X2=5.024,P<0.05), lymph node metastasis (X2=7.933, P<0.01) and neoplasm staging (X2=7.317,P<0.01), but not related to gender, age and tissue type (P>0.05). Slug was related to lymph node metastasis (X2=6.174,P<0.05) and neoplasm staging (X2=7.317,P<0.01), but not related to gender, age, tissue type and differentiation degree (P>0.05). Conclusion: The increase of NF-kB expression and Slug expression in NSCLC may be related to the generation, development and metastasis of NSCLC; in addition, NF-kB and Slug may synergistically inhibit E-cadherin expression, promote Vimentin expression and induce EMT of NSCLC, which further provides theoretical basis to the researches on EMT of NSCLC.
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