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目的：研究不同首次剂量匹罗卡品对氯化锂- 匹罗卡品颞叶癫痫大鼠模型诱发成功率、死亡率的影响。方法：90 只SD 大鼠随机分为三组，每组首次使用匹罗卡品剂量：A 组10mg/kg、B 组20mg/kg、C 组30mg/kg；随后采用多次注射10mg/kg 匹罗卡品，比较3 组癫痫持续状态诱发成功率、死亡率的差异。结果：A、B、C 组癫痫持续状态诱发成功率分别为66.7%、90%、93.3%。其中A 组与B 组之间诱发成功率差异有统计学意义，P < 0.05；C 组与B 组之间相比，差异无统计学意义，P >0.05。三组死亡率分别为 20%、23.3%、57.1%，其中A 组与B 组之间差异无统计学差异，P > 0.05，；C 组与其它两组相比较差异有统计学差异，P <0.05。结论：首次剂量20mg 匹罗卡品，然后10mg 多次反复注射，癫痫持续状态诱发成功率高、死亡率低，是一种理想的颞叶癫痫模型。

英文摘要:

Objective: We use different initial doses of pilocarpine after Lithium injections to establish the temporal lobe epilepsy models, observe the effects of different initial dose of pilocarpine on percentage of rats being induced successfully and the mortality rate. Methods: 90 SD rats were randomly divided into 3 groups, different initial doses of pilocarpine were used in every group: A group with 10mg/kg; B group with 20mg/kg and C group with 30mg/kg, followed later by repeated application of low dose pilocarpine 10 mg/kg,and observe the difference in percentage of rats exhibiting SE and the mortality rate between A, B and C. Results: Percentage of rats exhibiting SE of A, B and C group were 66.7%, 90%, 93.3% respectively. There was significant difference between A and B(P<0.05), but no difference between C and B (P>0.05). Mortality rate of A, B and C were 20%, 23.3%, 57.1% respectively; there was no difference between group A and group B(P>0.05), but group C showed difference in mortality rate with the other two groups(P<0.05). Conclusion: It is a good way to establish the temporal lobe epilepsy model with 20 mg initial dose of pilocarpine followed later by repeated application of 10 mg/kg pilocarpine, which could induce more rats exhibiting SE successfully and had lower mortality rate.

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